Li Yingcong, Zhu Benzhong, Xu Wentao, Zhu Hongliang, Chen Anjun, Xie Yuanhong, Shao Yi, Luo Yunbo
Laboratory of Fruit Physiology and Molecular Biology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People's Republic of China.
Plant Cell Rep. 2007 Nov;26(11):1999-2008. doi: 10.1007/s00299-007-0394-8. Epub 2007 Jul 18.
To study the function of LeERF1 in ethylene triple response on etiolated seedling, plant development and fruit ripening and softening, LeERF1 gene was introduced into tomato (Lycopersicon esculentum cv. No. 4 Zhongshu) through Agrobacterium-mediated transformation. The sense LeERF1 and anti-sense LeERF1 transgenic tomato were obtained. Overexpression of LeERF1 in tomato caused the typical ethylene triple response on etiolated seedling. In the adult stage, 35S::LeERF1 resulted in morphological changes in the leaves of the LeERF1-sn lines. Anti-sense LeERF1 fruits had longer shelf life compared with wild-type tomato. The results of this manuscript indicated that LeERF1 positively mediated the ethylene signals, while the function of LeERF1 was verified for the first time to be positively related with ethylene triple response on etiolated seedling, plant development and fruit ripening and softening using LeERF1-sn, wt and LeERF1-as tomato.
为研究LeERF1在黄化幼苗乙烯三重反应、植株发育以及果实成熟和软化过程中的功能，通过农杆菌介导的转化方法将LeERF1基因导入番茄（Lycopersicon esculentum cv. No. 4 Zhongshu）。获得了正义LeERF1和反义LeERF1转基因番茄。LeERF1在番茄中的过表达导致黄化幼苗出现典型的乙烯三重反应。在成年期，35S::LeERF1导致LeERF1-sn系叶片出现形态变化。与野生型番茄相比，反义LeERF1果实具有更长的货架期。本论文结果表明，LeERF1正向介导乙烯信号，并且利用LeERF1-sn、野生型和反义LeERF1番茄首次证实LeERF1的功能与黄化幼苗乙烯三重反应、植株发育以及果实成熟和软化呈正相关。
