College of Life Sciences, Zhejiang University, Hangzhou, China.
Water Sci Technol. 2012;65(5):795-801. doi: 10.2166/wst.2012.692.
Three bacterial strains, Arthrobacter sp. NB1, Serratia sp. NB2 and Stenotrophomonas sp. NB3, were isolated from contaminated sludge by using nitrobenzene as a sole source of carbon and nitrogen. It was observed that all three strains could degrade nitrobenzene at 400 mg/L initial concentration and mixed-cultivation of these strains could enhance the degradation of nitrobenzene compared with mono-cultivation. Mixture design was used for adjusting the proportions of each strain and the optimal ratio of inoculation size was NB1:NB2:NB3 = 4:4:5, where the nitrobenzene degradation percentage was two times higher than for by the single strain. The results of Plackett-Burman design indicated that Mg(2+), Ca(2+), Fe(2+), Zn(2+) and Mn(2+) had a positive effect on the degradation of nitrobenzene, while Cu(2+) and Co(2+) had a negative effect on it.
从被污染的污泥中，利用硝基苯作为唯一的碳源和氮源，分离出三株细菌，分别是节杆菌（Arthrobacter sp.）NB1、沙雷氏菌（Serratia sp.）NB2 和寡养单胞菌（Stenotrophomonas sp.）NB3。观察到所有三株细菌都能在初始浓度为 400mg/L 的硝基苯环境下进行降解，与单培养相比，混合培养这些菌株可以提高硝基苯的降解效率。通过使用混合设计来调整每个菌株的比例，发现最佳接种比例为 NB1:NB2:NB3=4:4:5，此时硝基苯的降解率比单菌株提高了两倍。Plackett-Burman 设计的结果表明，Mg(2+)、Ca(2+)、Fe(2+)、Zn(2+)和 Mn(2+)对硝基苯的降解有积极影响，而 Cu(2+)和 Co(2+)则有负面影响。
