Department of Environmental Engineering, College of Resources and Environmental Sciences, Nanjing Agricultural University, Weigang Road 1, Nanjing 210095, China.
Bioresour Technol. 2012 Apr;110:91-6. doi: 10.1016/j.biortech.2012.01.105. Epub 2012 Jan 28.
Strain NB1 was isolated from aerobic granular sludge capable of degrading nitrobenzene. Effects of several factors including pH, temperature, salinity, and second carbon/nitrogen source as co-substrate on the biodegradation of nitrobenzene by strain NB1 were investigated. Results showed that the optimal conditions for the biodegradation of nitrobenzene by strain NB1 were at pH 7.0 and 28-35°C. Under these conditions, the biodegradation rate of nitrobenzene could reach 9.29mgl(-1)h(-1) when the initial nitrobenzene concentration was 600mgl(-1). The addition of 1840mgl(-1) glucose or 500mgl(-1) ammonium chloride could slightly enhance the biodegradation efficiency, but even more addition of glucose or ammonium chloride delayed the biodegradation. Strain NB1 could degrade nitrobenzene in the presence of less than 3% NaCl (w/v %). Based on morphological and physiological characteristics as well as the phylogenetic analysis of 16S rDNA sequences, strain NB1 was identified as Klebsiella ornithinolytica.
NB1 菌株从能够降解硝基苯的好氧颗粒污泥中分离得到。考察了 pH 值、温度、盐度以及作为共基质的第二碳/氮源等因素对 NB1 菌株降解硝基苯的影响。结果表明，NB1 菌株降解硝基苯的最佳条件为 pH7.0 和 28-35°C。在这些条件下，当初始硝基苯浓度为 600mg/L 时，硝基苯的生物降解率可达 9.29mg/L·h-1。添加 1840mg/L 葡萄糖或 500mg/L 氯化铵可略微提高生物降解效率，但添加更多的葡萄糖或氯化铵会延迟生物降解。NB1 菌株可在小于 3%（w/v%）NaCl 的存在下降解硝基苯。根据形态学和生理学特征以及 16S rDNA 序列的系统发育分析，NB1 菌株被鉴定为鸟氨酸克雷伯氏菌。
