褪黑素对体外培养的大鼠甲状腺叶中[3H]-胸腺嘧啶核苷掺入DNA的影响。
Effects of melatonin on [3H]-thymidine incorporation into DNA of rat thyroid lobes in vitro.
作者信息
Wajs E, Lewiński A
机构信息
Laboratory of Thyrology, Institute of Endocrinology, University School of Medicine, Lódź, Poland.
出版信息
Biochem Biophys Res Commun. 1991 Dec 31;181(3):1187-91. doi: 10.1016/0006-291x(91)92064-q.
The effects of 4-h incubation in the presence of melatonin and N-acetylserotonin on the incorporation of [3H]-thymidine into DNA of the rat thyroid lobes were investigated. Additionally, thyroid lobes were incubated in the exposure to melatonin with thyrotropin jointly. Melatonin, when applied in the lowest concentration examined (10(-9)M), inhibited [3H]-thymidine incorporation, the effect of other concentrations (10(-6)M-10(-8)M) being not significant. N-acetylserotonin (10(-6)M) did not affect the rate of incorporation of that labelled nucleoside. As expected, thyrotropin enhanced the [3H]-thymidine incorporation into DNA of thyroid lobes. Melatonin (10(-7)M) suppressed that stimulatory effect of thyrotropin. The participation of melatonin in the regulation of thyroid growth processes is considered.
研究了褪黑素和N-乙酰血清素存在下4小时孵育对[3H]-胸腺嘧啶核苷掺入大鼠甲状腺叶DNA的影响。此外，甲状腺叶在褪黑素与促甲状腺激素共同作用下进行孵育。当以检测到的最低浓度（10(-9)M）应用褪黑素时，抑制了[3H]-胸腺嘧啶核苷的掺入，其他浓度（10(-6)M - 10(-8)M）的作用不显著。N-乙酰血清素（10(-6)M）不影响该标记核苷的掺入率。正如预期的那样，促甲状腺激素增强了[3H]-胸腺嘧啶核苷掺入甲状腺叶DNA。褪黑素（10(-7)M）抑制了促甲状腺激素的这种刺激作用。文中考虑了褪黑素在甲状腺生长过程调节中的作用。
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