生长抑素对体外培养的大鼠甲状腺叶基础状态及促甲状腺激素刺激下的³H-胸腺嘧啶核苷掺入的抑制作用。

Inhibitory effect of somatostatin on the basal and TSH-stimulated 3H-thymidine incorporation into rat thyroid lobes incubated in vitro.

作者信息

Zerek-Mełeń G, Lewiński A

机构信息

Department of Experimental Endocrinology and Hormone Diagnostic, Medical Academy of Lódź, Poland.

出版信息

Biochem Biophys Res Commun. 1988 Jul 15;154(1):417-20. doi: 10.1016/0006-291x(88)90702-4.

DOI:10.1016/0006-291x(88)90702-4

PMID:2899429

Abstract

The effects of somatostatin on the spontaneous and TSH--stimulated incorporation of tritiated thymidine into the rat thyroid lobes incubated in vitro were investigated. The rate of 3H-thymidine incorporation was used as an index of thyroid follicular cells (TFC) proliferation. It was shown that: 1) somatostatin, at a concentration of 10(-7)M, decreased 3H-thymidine incorporation into DNA of TFC, 2) the highest somatostatin concentration, as tested in this study (10(-6)M), produced a similar decreasing effect; the decrease, in this case, did not attain significance vs. controls, 3) somatostatin, when employed together with TSH, suppressed the stimulatory effect of the latter hormone on 3H-thymidine incorporation into DNA of thyroid lobes.

摘要

研究了生长抑素对体外培养的大鼠甲状腺叶中氚标记胸腺嘧啶核苷自发掺入及促甲状腺激素（TSH）刺激掺入的影响。3H-胸腺嘧啶核苷掺入率用作甲状腺滤泡细胞（TFC）增殖的指标。结果表明：1）浓度为10（-7）M的生长抑素可降低3H-胸腺嘧啶核苷掺入TFC的DNA中；2）本研究中测试的最高生长抑素浓度（10（-6）M）产生了类似的降低作用；但在这种情况下，与对照组相比，降低作用无统计学意义；3）生长抑素与TSH一起使用时，可抑制后者激素对甲状腺叶DNA中3H-胸腺嘧啶核苷掺入的刺激作用。