钠通道放大树突棘电位。
Sodium channels amplify spine potentials.
作者信息
Araya Roberto, Nikolenko Volodymyr, Eisenthal Kenneth B, Yuste Rafael
机构信息
Howard Hughes Medical Institute, Columbia University, New York, NY 10027, USA.
出版信息
Proc Natl Acad Sci U S A. 2007 Jul 24;104(30):12347-52. doi: 10.1073/pnas.0705282104. Epub 2007 Jul 18.
Abstract
Dendritic spines mediate most excitatory synapses in the brain. Past theoretical work and recent experimental evidence have suggested that spines could contain sodium channels. We tested this by measuring the effect of the sodium channel blocker tetrodotoxin (TTX) on depolarizations generated by two-photon uncaging of glutamate on spines from mouse neocortical pyramidal neurons. In practically all spines examined, uncaging potentials were significantly reduced by TTX. This effect was postsynaptic and spatially localized to the spine and occurred with uncaging potentials of different amplitudes and in spines of different neck lengths. Our data confirm that spines from neocortical pyramidal neurons are electrically isolated from the dendrite and indicate that they have sodium channels and are therefore excitable structures. Spine sodium channels could boost synaptic potentials and facilitate action potential backpropagation.
摘要
树突棘介导大脑中大多数兴奋性突触。过去的理论研究和最近的实验证据表明，树突棘可能含有钠通道。我们通过测量钠通道阻滞剂河豚毒素（TTX）对小鼠新皮层锥体神经元树突棘上谷氨酸双光子解笼产生的去极化的影响来对此进行测试。在几乎所有检查的树突棘中，TTX都显著降低了解笼电位。这种效应是突触后性的，并且在空间上局限于树突棘，并且在不同幅度的解笼电位以及不同颈部长度的树突棘中均会出现。我们的数据证实，新皮层锥体神经元的树突棘与树突在电上是隔离的，并表明它们具有钠通道，因此是可兴奋结构。树突棘钠通道可以增强突触电位并促进动作电位的回传。
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