Lee L F, Nazerian K, Leinbach S S, Reno J M, Boezi J A
J Natl Cancer Inst. 1976 Apr;56(4):823-7. doi: 10.1093/jnci/56.4.823.
Phosphonoacetate (PA), but not any of its analogues tested, effectively inhibited avian herpesvirus replication and viral DNA synthesis in cell cultures. At 100 mug/ml culture medium, PA completely inhibited the replication of Marek's disease virus (MDV), herpesvirus of turkeys, and owl herpesvirus, but had no measurable effect on normal cell growth. PA also inhibited DNA polymerases induced by these avian viruses. Enzyme inhibition was 50% at a PA concentration of 0.2 mug/ml. At a concentration of 3-6 mug/ml, the compound also effected a 50% inhibition of alpha (maxi) enzyme of the host DNA polymerase. It had no effect on the host beta (mini) enzyme. When administered to chickens, PA did not inhibit the replication of MDV, nor did it prevent the development of lymphoma.
膦乙酸（PA），而非其测试的任何类似物，可有效抑制细胞培养物中禽疱疹病毒的复制和病毒DNA合成。在100微克/毫升的培养基中，PA完全抑制马立克氏病病毒（MDV）、火鸡疱疹病毒和猫头鹰疱疹病毒的复制，但对正常细胞生长没有可测量的影响。PA还抑制这些禽病毒诱导的DNA聚合酶。在PA浓度为0.2微克/毫升时，酶抑制率为50%。在3 - 6微克/毫升的浓度下，该化合物还对宿主DNA聚合酶的α（最大）酶产生50%的抑制作用。它对宿主β（最小）酶没有影响。给鸡施用PA时，它既不抑制MDV的复制，也不阻止淋巴瘤的发展。
