Kim Hongtae, Huang Jun, Chen Junjie
Department of Therapeutic Radiology, Yale University School of Medicine, P.O. Box 208040, New Haven, Connecticut 06520, USA.
Nat Struct Mol Biol. 2007 Aug;14(8):710-5. doi: 10.1038/nsmb1277. Epub 2007 Jul 22.
The product of the breast cancer-1 gene, BRCA1, plays a crucial part in the DNA damage response through its interactions with many proteins, including BACH1, CtIP and RAP80. Here we identify a coiled-coil domain-containing protein, CCDC98, as a BRCA1-interacting protein. CCDC98 colocalizes with BRCA1 and is required for the formation of BRCA1 foci in response to ionizing radiation. Moreover, like BRCA1, CCDC98 has a role in radiation sensitivity and damage-induced G2/M checkpoint control. Together, these results suggest that CCDC98 is a mediator of BRCA1 function involved in the mammalian DNA damage response.
乳腺癌1基因（BRCA1）的产物通过与包括BACH1、CtIP和RAP80在内的多种蛋白质相互作用，在DNA损伤反应中发挥关键作用。在此，我们鉴定出一种含卷曲螺旋结构域的蛋白质CCDC98，它是一种与BRCA1相互作用的蛋白质。CCDC98与BRCA1共定位，并且是电离辐射诱导下BRCA1焦点形成所必需的。此外，与BRCA1一样，CCDC98在辐射敏感性和损伤诱导的G2/M期检查点控制中发挥作用。这些结果共同表明，CCDC98是参与哺乳动物DNA损伤反应的BRCA1功能的介导因子。
