Ahmad Ashfaq, Ali Haider, Khan Habiba, Begam Almas, Khan Sheraz, Ali Syed Shujait, Ahmad Naveed, Fazal Hina, Ali Mohammad, Hano Christophe, Ahmad Nisar, Abbasi Bilal Haider
Centre for Biotechnology and Microbiology, University of Swat, Swat 19200, Pakistan.
Department of Horticulture, The University of Agriculture, Peshawar 25120, Pakistan.
Plants (Basel). 2020 Mar 30;9(4):420. doi: 10.3390/plants9040420.
In current study, the effect of gibberellic acid was tested for production of biomass, polyphenolics and Steviol glycosides in adventitious root cultures of . Adventitious cultures were induced from the roots of in vitro grown plantlets on Murashige and Skoog (MS) medium containing combination of gibberellic acid (GA; 0.5, 1.0, 1.5 and 2.0 mg/L) and naphthalene acetic acid (NAA; 0.5 mg/L). Initially, a known mass of inoculum roots were shifted into suspension media augmented with various GA concentrations. The growth behavior of adventitious roots was recorded every 3 days for a period of 30 days. Maximum biomass biosynthesis (13.12 g/flask) was noticed in exponential phase on 27th day in the suspension containing 2.0 mg/L of GA. Other GA concentrations also displayed optimum patterns of biomass accumulation as compared to the control. Adventitious roots were investigated for total phenolic content (TPC) and production (TPP), total flavonoid content (TFC) and production (TFP), and 1, 1-diphenyl-2-picrylhydrazyl (DPPH)-based antioxidant potential. Maximum phenolics (TPC 9.84 mg gallic acid equivalent (GAE)/g-dry weight (DW)) and TPP (147.6 mg/L), TFC (5.12 mg Quercitin equivalent (QE)/g-DW) and TFP (76.91 mg/L) were observed in 2.0 mg/L GA treated cultures. The same concentration of gibberellic acid enhanced antioxidant activity (77.2%). Furthermore, maximum stevioside (7.13 mg/g-DW), rebaudioside-A (0.27 mg/g-DW) and dulcoside-A (0.001 mg/g-DW) were observed in roots exposed to 2.0 mg/L GA. This is the first report on the application of GA on biomass accumulation and secondary metabolite production in . The current study will be helpful to scale up the adventitious root cultures in bioreactors for the production of biomass and pharmaceutically important secondary metabolites.
在当前研究中，测试了赤霉素对甜叶菊不定根培养物中生物量、多酚和甜菊糖苷产量的影响。不定根培养物由体外生长的植株的根在含有赤霉素（GA；0.5、1.0、1.5和2.0 mg/L）和萘乙酸（NAA；0.5 mg/L）组合的Murashige和Skoog（MS）培养基上诱导产生。最初，将已知质量的接种根转移到添加了不同GA浓度的悬浮培养基中。每隔3天记录不定根的生长行为，持续30天。在含有2.0 mg/L GA的悬浮液中，第27天的指数期观察到最大生物量生物合成（13.12 g/瓶）。与对照相比，其他GA浓度也显示出最佳的生物量积累模式。对不定根进行了总酚含量（TPC）和产量（TPP）、总黄酮含量（TFC）和产量（TFP）以及基于1,1-二苯基-2-苦基肼（DPPH）的抗氧化潜力的研究。在2.0 mg/L GA处理的培养物中观察到最大酚类（TPC为9.84 mg没食子酸当量（GAE）/g干重（DW））和TPP（147.6 mg/L）、TFC（5.12 mg槲皮素当量（QE）/g-DW）和TFP（76.91 mg/L）。相同浓度的赤霉素增强了抗氧化活性（77.2%）。此外，在暴露于2.0 mg/L GA的根中观察到最大甜菊糖苷（7.13 mg/g-DW）、莱鲍迪苷A（0.27 mg/g-DW）和杜克苷A（0.001 mg/g-DW）。这是关于GA在甜叶菊生物量积累和次生代谢产物生产中的应用的首次报道。当前研究将有助于扩大生物反应器中不定根培养物的规模，以生产生物量和具有药学重要性的次生代谢产物。
