Vogt M, Bacheler L T, Boice L
J Virol. 1976 Mar;17(3):1009-26. doi: 10.1128/JVI.17.3.1009-1026.1976.
The various oligomeric viral DNA species produced at 32 C by two related syblines of ts-a-transformed mouse 3T3 cells were characterized. Results from the analysis of the cleavage products observed after digestion with restriction endonucleases from Haemophilus parainfluenzae, Escherichia coli RI, and Haemophilus suis are consistent with the assumption that in both sublines, the major oligomeric component is a dimer from which a segment of different length is deleted. The major oligomeric (27S) component in subline 1 was estimated to be 1.77 times the size of the viral monomer, and the major (25.5S) component in subline 15 was estimated to be 1.54 times the size of the viral monomer. These size estimates were confirmed by electron micrograph measurements. The larger oligomers produced by both sublines were found to be multiples of the major oligomeric component of each subline.
对在32℃下由ts-a转化的小鼠3T3细胞的两个相关亚系产生的各种寡聚体病毒DNA种类进行了表征。用副流感嗜血杆菌、大肠杆菌RI和猪嗜血杆菌的限制性内切酶消化后观察到的切割产物分析结果与以下假设一致：在两个亚系中，主要的寡聚体成分是一种二聚体，其中不同长度的片段被删除。亚系1中的主要寡聚体（27S）成分估计是病毒单体大小的1.77倍，亚系15中的主要（25.5S）成分估计是病毒单体大小的1.54倍。这些大小估计通过电子显微镜测量得到了证实。发现两个亚系产生的较大寡聚体是每个亚系主要寡聚体成分的倍数。
