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由嗜沫嗜血杆菌和流感嗜血杆菌H-I的限制酶产生的φX-174复制性DNA的片段图谱。

Fragment maps of phiX-174 replicative DNA produced by restriction enzymes from haemophilus aphirophilus and haemophilus influenzae H-I.

作者信息

Hayashi M N, Hayashi M

出版信息

J Virol. 1974 Nov;14(5):1142-51. doi: 10.1128/JVI.14.5.1142-1151.1974.

DOI:10.1128/JVI.14.5.1142-1151.1974
PMID:4610178
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC355631/
Abstract

phiX-174 replicative form (RF) DNA was cleaved by restriction enzymes from Haemophilus aphirophilus (Hap) and H. influenzae (H-I strain Osaka) (HinH). Five fragments were produced by Hap and eight by HinH. The positions of all of the Hap fragments and six of the HinH fragments were mapped on the genome by heteroduplex transfection and DNA-DNA hybridization methods. In addition, fragment sizes of phiX-174 RF DNA and S13 RF DNA by Hap and HinH were compared. Considerable differences of the size of the fragments produced from these closely related phage genomes were observed.

摘要

嗜沫嗜血杆菌（Hap）和流感嗜血杆菌（H-I株大阪株，HinH）的限制酶切割了φX-174复制型（RF）DNA。Hap产生了5个片段，HinH产生了8个片段。通过异源双链转染和DNA-DNA杂交方法，将所有Hap片段和6个HinH片段的位置定位在基因组上。此外，还比较了Hap和HinH对φX-174 RF DNA和S13 RF DNA的片段大小。观察到从这些密切相关的噬菌体基因组产生的片段大小存在显著差异。

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Fragment maps of phiX-174 replicative DNA produced by restriction enzymes from haemophilus aphirophilus and haemophilus influenzae H-I.由嗜沫嗜血杆菌和流感嗜血杆菌H-I的限制酶产生的φX-174复制性DNA的片段图谱。
J Virol. 1974 Nov;14(5):1142-51. doi: 10.1128/JVI.14.5.1142-1151.1974.
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引用本文的文献

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本文引用的文献

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Genetic Map of Bacteriophage phiX174.噬菌体φX174的遗传图谱
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