多瘤病毒DNA的研究：多瘤病毒基因组的切割图谱
Studies of polyoma virus DNA: cleavage map of the polyoma virus genome.
作者信息
Chen M C, Chang K S, Salzman N P
出版信息
J Virol. 1975 Jan;15(1):191-8. doi: 10.1128/JVI.15.1.191-198.1975.
Abstract
A small-plaque polyoma virus, MPC-1, was isolated from a mouse plasmacytoma. The DNA of this polyoma virus was cleaved with a restriction enzyme from Haemophilus influenzae (Hin d), and the molecular weights of the limit products were analyzed by electrophoresis and electron microscopy. The fragments produced by this enzyme have been ordered by analysis of partial digest products. A physical map of the polyoma virus genome was then constructed.
摘要
一种小斑块多瘤病毒，MPC - 1，是从一只小鼠浆细胞瘤中分离出来的。这种多瘤病毒的DNA用来自流感嗜血杆菌（Hin d）的一种限制性内切酶进行切割，并且通过电泳和电子显微镜分析了限制性产物的分子量。通过对部分消化产物的分析，已确定了该酶产生的片段顺序。随后构建了多瘤病毒基因组的物理图谱。
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