通过双相水性聚合物系统的分配平衡研究评估抗原-抗体亲和常数：更严格的分析
Evaluation of antigen-antibody affinity constants by partition equilibrium studies with a two-phase aqueous polymer system: a more rigorous analysis.
作者信息
Winzor D J
机构信息
Department of Biochemistry, University of Queensland, Brisbane, Australia.
出版信息
Biochim Biophys Acta. 1991 Dec 6;1115(2):141-4. doi: 10.1016/0304-4165(91)90022-9.
A quantitative procedure is devised for the determination of affinity constants from the composition-dependence of the antigen partition coefficient in liquid-liquid partition studies of antigen-antibody mixtures. Its use is illustrated by application to published results (Elling, L.R., Kula, M.-R., Hadas, E. and Katchalski-Katzir, E. (1991) Anal. Biochem. 192, 74-77) obtained for the interaction of horseradish peroxidase with an elicited monoclonal antibody by antigen distribution studies with a two-phase aqueous polymer system (poly(ethylene glycol)/dextran) for the partition analysis. Shortcomings in the original interpretation of these results are eliminated.
设计了一种定量方法，用于在抗原-抗体混合物的液-液分配研究中，根据抗原分配系数的组成依赖性来测定亲和常数。通过将其应用于已发表的结果（埃林，L.R.，库拉，M.-R.，哈达斯，E.和卡察尔斯基-卡齐尔，E.（1991年）《分析生物化学》192卷，74 - 77页）来说明该方法的用途，这些结果是通过使用双相水性聚合物体系（聚乙二醇/葡聚糖）进行抗原分布研究以分析辣根过氧化物酶与一种诱导产生的单克隆抗体的相互作用而获得的。消除了对这些结果原始解释中的不足之处。
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