Rolland-Sabaté Agnès, Colonna Paul, Mendez-Montealvo Maria Guadalupe, Planchot Véronique
Biopolymères Interactions Assemblages, INRA, F-44300 Nantes Cedex 03, France.
Biomacromolecules. 2007 Aug;8(8):2520-32. doi: 10.1021/bm070024z. Epub 2007 Jul 24.
The aim of this work was to characterize starch polysaccharides using asymmetrical flow field flow fractionation coupled with multiangle laser light scattering. Amylopectins from eight different botanical sources and rabbit liver glycogen were studied. Amylopectins and glycogen were completely solubilized and analyzed, and high mass recoveries were achieved (81.7-100.0%). Amylopectin Mw, RG, and the hydrodynamic coefficient nuG (the slope of the log-log plot of RGi vs Mi) were within the ranges 1.05-3.18 x 10(8) g mol(-1), 163-229 nm, 0.37-0.49, respectively. The data were also considered in terms of structural parameters. The results were analyzed by comparison with the theory of hyperbranched polymers (Flory, P. J. Principles of Polymer Chemistry; Cornell University Press: Ithaca, NY, 1953; Burchard, W. Macromolecules, 1977, 10, 919-927). This theory, based upon the ABC model, has been shown to underestimate the branching degrees of amylopectins. However, quantitative agreement with the data in the literature was found for amylopectins when using the ABC model modified by the introduction of a multiplying factor, determined from previously described amylopectin structures in terms of the number of branching point calculations.
这项工作的目的是使用不对称流场流分馏结合多角度激光光散射来表征淀粉多糖。研究了来自八种不同植物来源的支链淀粉和兔肝糖原。支链淀粉和糖原被完全溶解并进行分析，实现了较高的质量回收率（81.7 - 100.0%）。支链淀粉的分子量、回转半径（RG）和流体动力学系数nuG（RGi与Mi的对数-对数图的斜率）分别在1.05 - 3.18×10⁸ g·mol⁻¹、163 - 229 nm、0.37 - 0.49范围内。还从结构参数方面考虑了这些数据。通过与超支化聚合物理论（弗洛里，P. J.《聚合物化学原理》；康奈尔大学出版社：纽约伊萨卡，1953年；布尔查德，W.《大分子》，1977年，10卷，919 - 927页）进行比较来分析结果。基于ABC模型的该理论已被证明低估了支链淀粉的支化度。然而，当使用通过引入一个乘数因子进行修正的ABC模型时，发现支链淀粉与文献中的数据在定量上具有一致性，该乘数因子是根据先前描述的支链淀粉结构通过分支点计算数量来确定的。
