Wang Li-Wei, Li Qi, Hua Zhao-Lai, Zhou Fei, Keping Xie, Daoyan Wei, Yao James, Ajani Jaffer
Cancer Center, Shanghai Eastern Hospital, Tongfi University, Shanghai 200120, China.
Zhonghua Zhong Liu Za Zhi. 2007 Feb;29(2):107-11.
To investigate the expression of transcription factor Sp1 in gastric cancer tissue and its correlation with prognosis.
Sp1 expression patterns in specimens of 86 gastric cancers, 57 normal gastric tissues and 53 metastatic lymph nodes were detected by immunohistochemical method. The activity of Sp1 in the tumor and normal tissues was examined by electrophoretic mobility shift analysis (EMSA). The correlation between transcription factor Sp1 expression of tumors and patients' prognosis were statistically analyzed using Cox proportional hazard model.
In normal gastric tissue, Sp1 protein was predominantly expressed in the nuclei of cell located in the mucous neck region, but neither in the gastric pit cells with foveolar differentiation, nor in cells of the glandular epithelium with glandular differentiation. Strong Sp1 expression was also detected in tumor cells, but very weak or even no Sp1 expression in stromal cells or normal glandular cells surrounding the tumor. The median survival time of patients with negative, weak, and strong Sp1 expression was 43, 37, and 8 months, respectively (P < 0.01). Spl expression (P < 0.01) and stage (P < 0.001) were demonstrated as independent prognostic factor by multivariate analysis.
Normal and malignant gastric tissue are found to have its own unique Sp1 expression patterns. Sp1 expression may be used as an important survival predictor in human gastric cancer.
探讨转录因子Sp1在胃癌组织中的表达及其与预后的相关性。
采用免疫组织化学方法检测86例胃癌标本、57例正常胃组织标本及53例转移淋巴结中Sp1的表达情况。用电泳迁移率变动分析（EMSA）检测肿瘤组织和正常组织中Sp1的活性。采用Cox比例风险模型对肿瘤转录因子Sp1表达与患者预后的相关性进行统计学分析。
在正常胃组织中，Sp1蛋白主要表达于位于黏液颈部区域的细胞核中，而在具有小凹分化的胃小凹细胞及具有腺管分化的腺上皮细胞中均未表达。在肿瘤细胞中也检测到强Sp1表达，但在肿瘤周围的基质细胞或正常腺细胞中Sp1表达非常弱甚至无表达。Sp1表达阴性、弱阳性及强阳性患者的中位生存时间分别为43、37和8个月（P<0.01）。多因素分析显示，Sp1表达（P<0.01）和分期（P<0.001）是独立的预后因素。
正常和恶性胃组织具有各自独特的Sp1表达模式。Sp1表达可作为人类胃癌重要的生存预测指标。
