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噬菌体T5感染大肠杆菌后的早期事件。II. 噬菌体诱导的5'-核苷酸酶活性的调控

Early events after infection of Escherichia coli by bacteriophage T5. II. Control of the bacteriophage-induced 5'-nucleotidase activity.

作者信息

Berget S M, Mozer T J, Warner H R

出版信息

J Virol. 1976 Apr;18(1):71-9. doi: 10.1128/JVI.18.1.71-79.1976.

Abstract

The control of activity of the bacteriophage T5-induced 5'-nucleotidase is dependent upon the amount of T5 parental DNA injected into the cell and expressed. When only the first-step transfer DNA is injected and expressed the amount of 5'-nucleotidase activity observed is two to three times the maximum amount observed after normal T5 infection, and inactivation of the enzyme does not occur. Enzyme inactivation occurs only after the remaining DNA is injected, but only limited expression of this DNA is required. The control of the nucleotidase inactivation process is similar to that for the repair of the nicks in parental DNA, and is probably mediated by a class IIa protein.

摘要

噬菌体T5诱导的5'-核苷酸酶活性的控制取决于注入细胞并表达的T5亲本DNA的量。当仅注入并表达第一步转移DNA时,观察到的5'-核苷酸酶活性量是正常T5感染后观察到的最大量的两到三倍,并且酶不会失活。酶失活仅在剩余DNA注入后发生,但只需要该DNA的有限表达。核苷酸酶失活过程的控制与亲本DNA切口修复的控制相似,可能由IIa类蛋白介导。

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