噬菌体T5感染大肠杆菌后的早期事件。III.尿嘧啶-DNA糖基化酶活性的抑制
Early events after infection of Escherichia coli by bacteriophage T5. III. Inhibition of uracil-DNA glycosylase activity.
作者信息
Warner H R, Johnson L K, Snustad D P
出版信息
J Virol. 1980 Jan;33(1):535-8. doi: 10.1128/JVI.33.1.535-538.1980.
Abstract
The activity of uracil-DNA glycosylase in Escherichia coli decreases dramatically to less than 10% of its original level after infection of the cells by phage T5. Phage-induced protein synthesis is required for this inhibition to occur, and the inhibition is induced by a mutant capable of injecting only the first 8% of its DNA. The inhibitor activity in extracts of infected cells is heat labile and nondialyzable, and will inhibit enzyme activity present in extracts of uninfected cells.
摘要
在噬菌体T5感染大肠杆菌细胞后，大肠杆菌中尿嘧啶-DNA糖基化酶的活性急剧下降至其原始水平的不到10%。这种抑制作用的发生需要噬菌体诱导的蛋白质合成，并且这种抑制作用由一个只能注入其8% DNA的突变体诱导。感染细胞提取物中的抑制剂活性对热不稳定且不可透析，并且会抑制未感染细胞提取物中的酶活性。
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