Brok-Simoni F, Ashkenazi Y E, Ramot B, Holtzman F
Br J Haematol. 1976 Apr;32(4):601-7. doi: 10.1111/j.1365-2141.1976.tb00964.x.
The activities of glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP oxidoreductase, G6PD), 6-phosphogluconate dehydrogenase (6-phospho-d-gluconate: NADP oxidoreductase, 6PGD), hexokinase (ATP:D-hexose 6-phosphotransferase, HK), lactic dehydrogeanse (L-lactate: NAD oxidoreductase, LDH) and aspirate aminotransferase (L-aspartate: 2-oxoglutarate aminotransferase, Asp.T) were determined in red blood cells of 11 healthy individuals. The determinations were carried out on samples drawn every 4 h over a 24 h period. The activities of G6PD, 6PGD, LDH and Asp.T exhibited a semi-circadian rhythm, namely, two peaks of activity during 24 h while HK activity demonstrated a true circadian rhythm. In addition a polymorphism of the G6PD and LDH activity patterns was observed. The implications of a biological clock in enucleated cells are discussed.
测定了11名健康个体红细胞中葡萄糖-6-磷酸脱氢酶（D-葡萄糖-6-磷酸：NADP氧化还原酶，G6PD）、6-磷酸葡萄糖酸脱氢酶（6-磷酸-D-葡萄糖酸：NADP氧化还原酶，6PGD）、己糖激酶（ATP：D-己糖6-磷酸转移酶，HK）、乳酸脱氢酶（L-乳酸：NAD氧化还原酶，LDH）和天冬氨酸转氨酶（L-天冬氨酸：2-酮戊二酸氨基转移酶，Asp.T）的活性。在24小时内每4小时采集一次样本进行测定。G6PD、6PGD、LDH和Asp.T的活性呈现半昼夜节律，即在24小时内有两个活性峰值，而HK活性呈现真正的昼夜节律。此外，还观察到G6PD和LDH活性模式的多态性。讨论了生物钟在无核细胞中的意义。
