[骨膜蛋白在增生性瘢痕中的表达及其与转化生长因子-β1及其受体的关系]
[The expression of periostin in hyperplasic scars and the relations to TGF-beta1 and its receptors].
作者信息
Wang Qi, Nie Fang-Fei, Zhao Xia, Qin Ze-Lian
机构信息
Department of Plastic Surgery, Third Hospital of Pecking University, Bejing 100083, China.
出版信息
Zhonghua Zheng Xing Wai Ke Za Zhi. 2007 May;23(3):229-32.
OBJECTIVE
To probe into periostin's role in the pathological mechanism of hyperplasic scars, by examining the expression of periostin in hyperplasic scar tissues. To investigate the correlations between periostin and TGF-beta1, TGF-beta R I, TGF-beta R II.
METHODS
RT-PCR was used to assess the mRNA expression levels of TGF-beta1, TGF-beta R I, TGF-beta R II in three kinds of tissues, which are keloid (K), hypertrophic scar (HS) and normal skin (SK). The protein expression of periostin was measured with Western blotting.
RESULTS
The mRNA level of periostin in K was higher than that in SK. The mRNA expression of TGF-beta1 in K was higher than that in HS and SK. The mRNA level of TGF-beta R I in K was higher than that in HS and SK. The significances above all was at P < 0.01. The protein expression level of periostin in HS increased, compared with that in SK (P < 0.05). Periostin was related to TGF-beta1 positively (P <0.01).
CONCLUSIONS
The periostin's expression is increased in keloids. Periostin is a cicatrix specific gene. Periostin appears to play an important role in the formation of keloids, which is related to TGF-beta1 closely.
目的
通过检测增生性瘢痕组织中骨膜蛋白的表达，探讨骨膜蛋白在增生性瘢痕病理机制中的作用。研究骨膜蛋白与转化生长因子-β1（TGF-β1）、转化生长因子-βⅠ型受体（TGF-βRⅠ）、转化生长因子-βⅡ型受体（TGF-βRⅡ）之间的相关性。
方法
采用逆转录聚合酶链反应（RT-PCR）检测瘢痕疙瘩（K）、增生性瘢痕（HS）和正常皮肤（SK）三种组织中TGF-β1、TGF-βRⅠ、TGF-βRⅡ的mRNA表达水平。用蛋白质印迹法检测骨膜蛋白的蛋白表达。
结果
K中骨膜蛋白的mRNA水平高于SK。K中TGF-β1的mRNA表达高于HS和SK。K中TGF-βRⅠ的mRNA水平高于HS和SK。上述所有差异均具有统计学意义（P<0.01）。与SK相比，HS中骨膜蛋白的蛋白表达水平升高（P<0.05）。骨膜蛋白与TGF-β1呈正相关（P<0.01）。
结论
瘢痕疙瘩中骨膜蛋白的表达增加。骨膜蛋白是一种瘢痕特异性基因。骨膜蛋白在瘢痕疙瘩的形成中似乎起重要作用，且与TGF-β1密切相关。
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