Allison D G, Sattenstall M A
School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Oxford Road, Manchester, England, UK.
J Appl Microbiol. 2007 Aug;103(2):318-24. doi: 10.1111/j.1365-2672.2006.03243.x.
To investigate the effect of green flourescent protein (GFP) incorporation on bacterial physiology.
Comparisons were made between four different isogenic pairings of non-GFP-containing parents and their GFP-containing transformants with respect to growth rate and antimicrobial susceptibility. For the latter, sensitivities to 12 different antibiotics were measured initially by disc-diffusion assay, and then subsequently by generation of dose-dependent survival curves for 1 h exposure to different concentrations of tetracycline, ciprofloxacin and cetrimide USP. Whilst no significant difference in growth rate was observed, GFP-containing strains were uniformly and significantly more sensitive to all antimicrobial agents tested, excluding the beta-lactams, than their respective non-GFP-containing counterparts.
GFP incorporation has a significant effect on bacterial physiology and can modulate antimicrobial susceptibility.
Transformation with GFP can affect the physiology of bacterial cells. This may therefore affect the quality and accuracy of data generated depending on the application for which GFP is used.
研究绿色荧光蛋白（GFP）掺入对细菌生理学的影响。
对四组不同的不含GFP的亲本及其含GFP的转化体的同基因配对进行了生长速率和抗菌药敏性比较。对于后者，最初通过纸片扩散法测定对12种不同抗生素的敏感性，随后通过生成不同浓度四环素、环丙沙星和西曲溴铵USP暴露1小时的剂量依赖性存活曲线进行测定。虽然未观察到生长速率有显著差异，但与各自不含GFP的对应菌株相比，含GFP的菌株对所有测试抗菌剂（不包括β-内酰胺类）均一致且显著更敏感。
GFP掺入对细菌生理学有显著影响，并可调节抗菌药敏性。
用GFP转化可影响细菌细胞的生理学。因此，这可能会影响根据GFP的使用用途所产生数据的质量和准确性。
