Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Science, 02-109 Warsaw, Poland.
J Microbiol Biotechnol. 2010 Jan;20(1):224-8.
The encapsulation of bacteria may be used to harness them for longer period of time in order to make them viable, while antibiotic treatment would result in controlled release of therapeutic molecules. Encapsulated bacteria Escherichia coli GFP (green fluorescent protein) (E. coli GFP) were used here as a model for therapeutic substance - GFP fragments release (model of bioactive substances). Our aim was to evaluate the performance of bacteria encapsulated in hollow fibers (HF) treated with antibiotic for induction of cell death. The polypropylene surface modified HF was applied for E. coli encapsulation. The encapsulated bacteria were treated with tetracycline in vitro or in vivo during subcutaneous implantation into mice. The HF content was evaluated in flow cytometer, to assess the bacteria cell membrane permeability changes induced by tetracycline treatment. It was observed that applied membranes prevent release of bacteria through the HF wall. The encapsulated in HF E. coli GFP culture in vitro proves the tetracycline impact on bacteria viability and allows recognition sequence of events within process of bacteria death. Treatment with tetracycline of the SCID mice for 8 hours proves the tetracycline impact on bacteria viability in vivo, rising the necrotic bacteria releasing GFP fragments. It was concluded, that the bacteria may be safely enclosed within the HF at site of implantation, and when the animal is treated with antibiotic bacteria may act as a local source of bioactive factor.
细菌的封装可以用于在更长的时间内控制它们的生存能力，而抗生素治疗则会导致治疗分子的受控释放。在这里，我们使用封装的细菌大肠杆菌 GFP（绿色荧光蛋白）（E. coli GFP）作为治疗物质 - GFP 片段释放的模型（生物活性物质模型）。我们的目的是评估用抗生素处理的中空纤维（HF）中封装的细菌的性能，以诱导细胞死亡。用聚丙烯表面改性 HF 进行大肠杆菌封装。将封装的细菌用四环素进行体外或体内处理，然后皮下植入小鼠。用流式细胞仪评估 HF 内容物，以评估四环素处理引起的细菌细胞膜通透性变化。结果表明，应用的膜可以防止细菌通过 HF 壁释放。在体外培养的 HF 中封装的 GFP 大肠杆菌证明了四环素对细菌活力的影响，并允许识别细菌死亡过程中的事件序列。用四环素处理 SCID 小鼠 8 小时证明了体内四环素对细菌活力的影响，导致坏死细菌释放 GFP 片段。结论是，细菌可以在植入部位的 HF 内安全地封闭，当动物用抗生素治疗时，细菌可以作为局部生物活性因子的来源。
