参松养心胶囊干粉溶液对豚鼠心室肌细胞钠电流和L型钙电流的影响：实验研究

[Effects of solution of dry powder of ShenSongYangXin capsule on sodium current and L-type calcium current in ventricular myocytes: experiment with guinea pig].

作者信息

Li Ning, Huo You-ping, Ma Ke-juan, Sun Qi, Pu Jie-lin

机构信息

Center for Arrhythmia Diagnosis and Treatment, Fuwai Cardiovascular Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100037, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2007 Apr 10;87(14):995-8.

PMID:17650428

Abstract

OBJECTIVE

To investigate the effects of dry powder of ShenSongYangXin capsule on sodium current (I(Na)) and L-type calcium current (I(Ca, L)) in ventricular myocytes.

METHODS

Ventricular myocytes were isolated from guinea pig. Solution of the dry powder of ShenSongYangXin capsule of the concentrations of 1%, 0.5%, and 0.25% was added into the suspension of the myocytes. Whole cell patch-clamp technique was used to detect the I(Na) and I(Ca, L) in the ventricular myocytes.

RESULT

ShenSongYangXin decreased the peak I(Na) from 27.2 +/- 5.4 (pA/pF) to 14.9 +/- 2.8 (pA/pF), with a decrease rate of 44.8% +/- 7.7% (n = 5, P < 0.05). The solution of dry powder of ShenSongYangXin of the concentrations of 1%, 0.5%, and 0.25% decreased the peak L-type calcium channel current (I(ca, L)) significantly in a concentration dependent manner with the reduction rates of 50.7% +/- 5.6%, 44.8% +/- 6.5%, and 19.2% +/- 1.1% respectively (n = 5, all P < 0.05). ShenSongYangXin shifted up both the I approximately 1V curve of I(Na) and that of I(Ca, L), while their active, peak and reverse potentials didn't change.

CONCLUSION

ShenSongYangXin blocks both I(Na) and I(Ca, L), which may contributes to some of its antiarrhythmic effect.

摘要

目的

研究参松养心胶囊干粉对心室肌细胞钠电流（I(Na)）和L型钙电流（I(Ca,L)）的影响。

方法

从豚鼠分离心室肌细胞。将浓度为1%、0.5%和0.25%的参松养心胶囊干粉溶液加入到心肌细胞悬液中。采用全细胞膜片钳技术检测心室肌细胞中的I(Na)和I(Ca,L)。

结果

参松养心使I(Na)峰值从27.2±5.4（pA/pF）降至14.9±2.8（pA/pF），下降率为44.8%±7.7%（n = 5，P < 0.05）。浓度为1%、0.5%和0.25%的参松养心胶囊干粉溶液以浓度依赖性方式显著降低L型钙通道电流（I(ca,L)）峰值，降低率分别为50.7%±5.6%、44.8%±6.5%和19.2%±1.1%（n = 5，均P < 0.05）。参松养心使I(Na)和I(Ca,L)的I-V曲线均上移，而它们的激活、峰值和反转电位未改变。