Zhang Di, Ding Yi
Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences,Wuhan University, Wuhan 430072, China.
Yi Chuan. 2007 Jun;29(6):725-30. doi: 10.1360/yc-007-0725.
Seven primer combinations were used for AFLP analysis. The fingerprints of 36 wild close relatives of barley in Tibet of China and 4 cultivated varieties of barley had been obtained, bringing about 227 discernable bands. Among the bands 194 were polymorphic with the percentage of 85.46%, indicating that the materials in the test have abundant genetic diversity. The genetic distance (Euclidean Distance) of samples computed by SPSS is between 2.646 and 10.488. Applying Ward's method to cluster the AFLP data results, an AFLP dendrogram of 40 barley materials was then established. All samples were clustered into 5 groups, further revealing the similarity, complicacy of the genetic background and the relationship of phylogenesis of the barley materials tested. Consulting the genetic identity it was shown that six-rowed wild close barley is closer to cultivated barley than two-rowed wild close barley. This result supported the viewpoint that the six-rowed wild close barley was the intermediate type in the evolutionary route from the tow-rowed wild close barley to the cultivated barley.
使用七种引物组合进行AFLP分析。已获得中国西藏36份野生近缘大麦和4个栽培大麦品种的指纹图谱，共产生227条可分辨条带。其中194条为多态性条带，多态性比例为85.46%，表明供试材料具有丰富的遗传多样性。通过SPSS计算的样本遗传距离（欧氏距离）在2.646至10.488之间。应用Ward法对AFLP数据结果进行聚类，构建了40份大麦材料的AFLP聚类图。所有样本被分为5组，进一步揭示了供试大麦材料遗传背景的相似性、复杂性及系统发育关系。通过遗传相似性分析表明，六棱野生近缘大麦比二棱野生近缘大麦更接近栽培大麦。这一结果支持了六棱野生近缘大麦是二棱野生近缘大麦向栽培大麦进化过程中的中间类型这一观点。
