Ozkan Hakan, Kafkas Salih, Sertac Ozer M, Brandolini Andrea
Department of Field Crops, Faculty of Agriculture, University of Cukurova, 01330 Adana, Turkey.
Theor Appl Genet. 2005 Dec;112(1):12-20. doi: 10.1007/s00122-005-0067-3. Epub 2005 Nov 8.
To assess the genetic diversity and the genetic structure of Turkish wild barley (Hordeum spontaneum Tell.) populations, 76 genotypes from ten ecologically and geographically different locations were analyzed by means of amplified fragment length polymorphism (AFLP) markers. Five primer combinations produced 187 scorable bands, of which 117 (62.6%) were polymorphic. Several population-specific and genotype-specific bands were identified, which differentiate populations or genotypes. Genetic distance, determined by Nei's distance coefficient, varied from 0.07 to 0.21 with an average of 0.13. In the UPGMA dendrogram based on Nei genetic distances, the Hordeum spontaneum populations were separated into two major clusters. Genetic diversity was larger among (68%) than within (32%) populations. Eight AFLP bands were strongly correlated to the altitude of the collecting site, while no clear trend was detected between geographical origin and genetic diversity. Our results strongly suggest the need for a change in Hordeum spontaneum sampling strategy: more populations, rather then more individuals within population, should be sampled to appraise and safeguard genetic diversity in the wild barley gene pool.
为评估土耳其野生大麦（Hordeum spontaneum Tell.）种群的遗传多样性和遗传结构，利用扩增片段长度多态性（AFLP）标记对来自十个生态和地理不同地点的76个基因型进行了分析。五种引物组合产生了187条可计分条带，其中117条（62.6%）具有多态性。鉴定出了几条种群特异性和基因型特异性条带，这些条带可区分不同种群或基因型。通过Nei距离系数确定的遗传距离在0.07至0.21之间，平均为0.13。在基于Nei遗传距离的UPGMA聚类图中，野生大麦种群被分为两个主要类群。种群间的遗传多样性（68%）大于种群内的遗传多样性（32%）。八条AFLP条带与采集地点的海拔高度密切相关，而地理起源与遗传多样性之间未检测到明显趋势。我们的结果强烈表明需要改变野生大麦的采样策略：应采集更多的种群，而不是在种群内采集更多的个体，以评估和保护野生大麦基因库中的遗传多样性。
