Tulin Edgardo E, Ecleo Zenaida T
PhilRootcrops, Leyte State University, Visca, Baybay, Leyte, Philippines.
J Med Food. 2007 Jun;10(2):290-9. doi: 10.1089/jmf.2006.067.
This study evaluated Philippine indigenous plants for cytokine-mimetic properties and partially characterized candidate mimetics for their stability as well as their sensitivity to variations in temperature and pH. Forty-five plant extract preparations were tested for their ability to stimulate proliferation of mouse spleen cells and bone marrow cells. Temperature and pH effects were determined by subjecting the extracts to different temperature and pH levels and measuring their residual proliferative activities. Results revealed that 24 and 14 extracts were able to stimulate proliferation of bone marrow cells and spleen cells, respectively. Extracts from yam (Dioscorea alata L.) (Dioscoreaceae) roots and leaves, taro (Colocasia esculenta L.) (Araceae) roots, and buyo (Piper betle L.) (Piperaceae) leaf were among the extracts that strongly stimulated proliferation of both bone marrow cells and splenocytes, significantly increasing cell concentrations. Heating the extracts beyond 40 degrees C markedly reduced their proliferative ability, while a pH level below 4.0 and above 8.0 also significantly decreased the extracts' activity. Three protein-mimetics with sizes of 26 kDa, 35 kDa, and 50 kDa were isolated from buyo leaf, yam root, and taro root extracts, respectively. All three molecules are biologically active and stimulate a dose-dependent proliferative response.
本研究评估了菲律宾本土植物的细胞因子模拟特性，并对候选模拟物的稳定性及其对温度和pH值变化的敏感性进行了部分表征。对45种植物提取物制剂进行了测试，以检测它们刺激小鼠脾细胞和骨髓细胞增殖的能力。通过将提取物置于不同的温度和pH水平并测量其残余增殖活性来确定温度和pH的影响。结果显示，分别有24种和14种提取物能够刺激骨髓细胞和脾细胞的增殖。山药（薯蓣科，参薯）根和叶、芋头（天南星科，芋）根以及蒌叶（胡椒科，蒌叶）叶的提取物是强烈刺激骨髓细胞和脾细胞增殖、显著增加细胞浓度的提取物之一。将提取物加热至40摄氏度以上会显著降低其增殖能力，而pH值低于4.0和高于8.0也会显著降低提取物的活性。分别从蒌叶、山药根和芋头根提取物中分离出了大小为26 kDa、35 kDa和50 kDa的三种蛋白质模拟物。这三种分子均具有生物活性，并能刺激剂量依赖性增殖反应。
