Lin Pei-Lan, Lin Kuo-Wei, Weng Ching-Feng, Lin Kuo-Chih
Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Hualien County 974, Taiwan, Republic of China.
J Agric Food Chem. 2009 Jun 10;57(11):4606-13. doi: 10.1021/jf8038499.
The aim of this study was to elucidate the effect of the major storage protein dioscorin isolated from two different yam species, Tainong No. 1 (TN1-dioscorins) and Japanese yam (Dj-dioscorins), on the immune activities of mice. Dj-dioscorins, like TN1-dioscorins, could induce expression of the pro-inflammatory cytokines and stimulate phagocytosis of RAW 264.7. Intraperitoneal injection of the TN1-dioscorins into mice stimulated phagocytosis of bone marrow, spleen, and thymic cells. In contrast, the T and B cells in bone marrow, spleen, and thymus isolated from mice injected with Dj-dioscorins had higher proliferative responses to mitogens. Furthermore, Dj-dioscorins enhanced proliferation of CD4(+), CD8(+), and Tim3(+) (Th1) cells in spleen and CD19(+) cells in both spleen and thymus. Supplement of Dj-dioscorins in the lymphoid cells isolated from Dj-dioscorins primed mice induced cell proliferation of both spleen and thymic cells. These findings indicated that TN1-dioscorins have a higher ability to stimulate the phagocytic activity of the lymphoid cells than Dj-dioscorins, whereas Dj-dioscorins possess more abilities than TN1-dioscorins to enhance the proliferation of the lymphoid cells.
本研究的目的是阐明从两种不同山药品种，即台农1号（TN1 - 薯蓣球蛋白）和日本山药（Dj - 薯蓣球蛋白）中分离出的主要贮藏蛋白薯蓣球蛋白对小鼠免疫活性的影响。与TN1 - 薯蓣球蛋白一样，Dj - 薯蓣球蛋白可诱导促炎细胞因子的表达并刺激RAW 264.7的吞噬作用。向小鼠腹腔注射TN1 - 薯蓣球蛋白可刺激骨髓、脾脏和胸腺细胞的吞噬作用。相比之下，从注射了Dj - 薯蓣球蛋白的小鼠中分离出的骨髓、脾脏和胸腺中的T细胞和B细胞对有丝分裂原具有更高的增殖反应。此外，Dj - 薯蓣球蛋白增强了脾脏中CD4(+)、CD8(+)和Tim3(+)（Th1）细胞以及脾脏和胸腺中CD19(+)细胞的增殖。从用Dj - 薯蓣球蛋白致敏的小鼠中分离的淋巴细胞中补充Dj - 薯蓣球蛋白可诱导脾脏和胸腺细胞的细胞增殖。这些发现表明，TN1 - 薯蓣球蛋白比Dj - 薯蓣球蛋白具有更高的刺激淋巴细胞吞噬活性的能力，而Dj - 薯蓣球蛋白比TN1 - 薯蓣球蛋白具有更强的增强淋巴细胞增殖能力。
