Zizka Jan, Hrdý Jirí, Lodinová-Zádníková Raja, Kocourková Ingrid, Novotná Olga, Sterzl Ivan, Prokesová Ludmila
1st Faculty of Medicine, Institute of Immunology and Microbiology, Charles University, Prague, Czech Republic.
Pediatr Allergy Immunol. 2007 Sep;18(6):486-94. doi: 10.1111/j.1399-3038.2007.00563.x. Epub 2007 Jul 25.
Maternal milk has beneficial effects on the development and function of the newborn's immune system. Whether the milk of allergic mother has the same effects as the milk of healthy mothers is not yet quite clear. To contribute to the characterization of its immunomodulatory action, we tested the effect of milk of healthy and allergic mothers on the proliferation and immunoglobulin formation in cultures of cord blood mononuclear leucocytes (CBML) of newborns of healthy and allergic mothers. CBML proliferation was tested by (3)H-thymidine incorporation, IgM, IgG and IgA production by reverse ELISPOT. CBML response was examined in unstimulated cultures and after stimulation with polyclonal activators in the presence or absence of colostrum or milk. The cells of children of allergic mothers have a significantly higher proliferative activity than those of children of healthy mothers. Maternal colostrum/milk in high doses markedly suppresses cell proliferation after stimulation with polyclonal activators, whereas lower milk doses in the cultures have no such effect and exert a rather stimulatory action. Immunoglobulin production by cord blood lymphocytes is also different in the two groups of children. Low basal immunoglobulin formation is increased after stimulation with a strong polyclonal activator of B cells--Bacillus firmus, CBML of children of allergic mothers produce more IgA than those of children of healthy mothers. The stimulated production of all immunoglobulin classes in cells of children of healthy mothers is still enhanced by colostrum/milk. Children of allergic mothers show a markedly increased production of only IgM and IgA. The effect of healthy and allergic colostrum and milk on cell proliferation and immunoglobulin production is similar. The lymphocytes of children of allergic mothers differ from the lymphocytes of children of healthy mothers in their proliferative activity and the ability to form immunoglobulin already at birth.
母乳对新生儿免疫系统的发育和功能具有有益作用。过敏母亲的乳汁是否与健康母亲的乳汁具有相同作用尚不完全清楚。为了有助于表征其免疫调节作用，我们测试了健康母亲和过敏母亲的乳汁对健康母亲和过敏母亲新生儿的脐血单个核白细胞（CBML）培养物中细胞增殖和免疫球蛋白形成的影响。通过³H-胸腺嘧啶核苷掺入法检测CBML增殖，通过反向ELISPOT检测IgM、IgG和IgA的产生。在未刺激的培养物中以及在有或没有初乳或乳汁存在的情况下用多克隆激活剂刺激后，检测CBML反应。过敏母亲的孩子的细胞比健康母亲的孩子的细胞具有明显更高的增殖活性。高剂量的母体初乳/乳汁在用多克隆激活剂刺激后可显著抑制细胞增殖，而培养物中较低剂量的乳汁则没有这种作用，反而具有刺激作用。两组儿童的脐血淋巴细胞产生免疫球蛋白的情况也有所不同。低基础免疫球蛋白形成在受到B细胞的强多克隆激活剂——坚强芽孢杆菌刺激后会增加，过敏母亲的孩子的CBML产生的IgA比健康母亲的孩子的CBML产生的IgA更多。初乳/乳汁仍可增强健康母亲的孩子的细胞中所有免疫球蛋白类别的刺激产生量。过敏母亲的孩子仅显示IgM和IgA的产生量明显增加。健康和过敏初乳及乳汁对细胞增殖和免疫球蛋白产生的影响相似。过敏母亲的孩子的淋巴细胞在增殖活性和出生时形成免疫球蛋白的能力方面与健康母亲的孩子的淋巴细胞不同。
