Miyagawa Y, Sugita K, Komiyama A, Akabane T
Pediatrics. 1980 Mar;65(3):497-500.
Pokeweed mitogen-induced immunoglobulin (Ig) production by cord lymphocytes was studied in vitro by Ig-secreting plaque-forming cell (Ig-PFC) assay. Although adult mononuclear cells generated all of IgM-, IgG-, and IgA-PFC, cord mononuclear cells generated only IgM-PFC when cultured for seven days. The number of cord IgM-PFC was 102 +/- 26/10(4) mononuclear cells, being about one fourth of that of adult IgM-PFC. When cultured for 14 days, cord mononuclear cells formed increased numbers of IgM-PFC in contrast to adult cells and yielded IgG-PFC as well, indicating delayed Ig production. Cord T cells were much less effective at helping adult B cells to differentiate into Ig-PFC as compared with adult T cells. Substitution of adult T cells for cord T cell markedly improved the response of cord B cells. The present study demonstrates Ig secretion by cord lymphocytes in response to pokeweed mitogen stimulation. The results further indicate that the delayed Ig production by cord lymphocytes is largely due to functional immaturity of the T cells.
采用分泌免疫球蛋白的空斑形成细胞（Ig-PFC）分析法，在体外研究了商陆丝裂原诱导脐血淋巴细胞产生免疫球蛋白（Ig）的情况。尽管成人单核细胞可产生所有的IgM、IgG和IgA-PFC，但脐血单核细胞培养7天时仅产生IgM-PFC。脐血IgM-PFC的数量为102±26/10⁴单核细胞，约为成人IgM-PFC数量的四分之一。培养14天时，与成人细胞相比，脐血单核细胞形成的IgM-PFC数量增加，并且还产生了IgG-PFC，这表明Ig产生延迟。与成人T细胞相比，脐血T细胞在帮助成人B细胞分化为Ig-PFC方面的效果要差得多。用成人T细胞替代脐血T细胞可显著改善脐血B细胞的反应。本研究证明了脐血淋巴细胞在商陆丝裂原刺激下可分泌Ig。结果进一步表明，脐血淋巴细胞Ig产生延迟主要是由于T细胞功能不成熟。
