Van Der Straeten D, Rodrigues Pousada R A, Gielen J, Van Montagu M
Laboratorium voor Genetica, Universiteit Gent, Belgium.
FEBS Lett. 1991 Dec 16;295(1-3):39-42. doi: 10.1016/0014-5793(91)81379-m.
We have isolated and sequenced a partial tomato alcohol dehydrogenase (Adh) cDNA clone. Expression of tomato Adh was studied at the messenger RNA level in seedlings, roots, and fruit. High induction was observed under hypoxic conditions, both in tomato seedlings and in roots. In addition, the Adh mRNA was present at the mature green and pink stage of the tomato fruit, and was highly induced in late ripening. Moreover, an artificial ripening treatment resulted in at least 50-fold induction compared to the mature green mRNA level. Genomic DNA gel blotting suggested the presence of a multigene family for Adh in tomato.
我们分离并测序了一个番茄乙醇脱氢酶（Adh）的部分cDNA克隆。在信使RNA水平上研究了番茄Adh在幼苗、根和果实中的表达情况。在低氧条件下，番茄幼苗和根中均观察到高诱导表达。此外，Adh mRNA在番茄果实的绿熟期和粉红期存在，并在成熟后期高度诱导表达。而且，与绿熟期mRNA水平相比，人工催熟处理导致至少50倍的诱导表达。基因组DNA凝胶印迹分析表明番茄中存在Adh多基因家族。
