Xie Y, Wu R
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853.
Plant Mol Biol. 1989 Jul;13(1):53-68. doi: 10.1007/BF00027335.
Anaerobiosis rapidly induces alcohol dehydrogenase (ADH), an enzyme of the fermentation pathway, in different parts of rice seedlings. After initiation of anaerobiosis, the activity of the enzyme increases linearly for 3 days or more. The ADH activity is anaerobically inducible even in mature rice leaves in contrast to maize which shows no induction in mature leaves. Rice ADH activity can also be induced by an auxin analog, 2,4-dichlorophenoxyacetic acid, under aerobic conditions. The experimental results show that anaerobiosis increases the ADH mRNA level, indicating that the ADH enzyme is regulated at the transcriptional level. Starch gel electrophoresis of a protein extract from rice shows 3 distinct forms of ADH. The amounts of the 3 forms vary with the organ, suggesting that the expression of ADH genes is organ-specific. Sequencing data show that the two different cloned cDNA copies of ADH mRNAs are derived from two different genes.
缺氧迅速诱导水稻幼苗不同部位的乙醇脱氢酶（ADH），这是一种发酵途径的酶。缺氧开始后，该酶的活性会线性增加3天或更长时间。与玉米在成熟叶片中无诱导现象不同，即使在成熟水稻叶片中，ADH活性也可被厌氧诱导。在有氧条件下，水稻ADH活性也可被生长素类似物2,4-二氯苯氧乙酸诱导。实验结果表明，缺氧会增加ADH mRNA水平，这表明ADH酶在转录水平上受到调控。水稻蛋白质提取物的淀粉凝胶电泳显示出3种不同形式的ADH。这3种形式的含量因器官而异，表明ADH基因的表达具有器官特异性。测序数据表明，ADH mRNA的两个不同克隆cDNA拷贝来自两个不同的基因。
