Asante E A, Hill W G, Bulfield G
Cellular and Molecular Biology Department, AFRC Institute of Animal Physiology and Genetics, Edinburgh Research Station, Roslin, Midlothian.
Genet Res. 1991 Oct;58(2):123-7. doi: 10.1017/s0016672300029773.
The flux through the de novo fatty acid synthesis pathway was estimated in lines of mice which differed substantially in fat content following 26 generations of selection at 10 weeks of age. Previous estimates of lipogenic enzyme activities had indicated an increase in the capacity for lipogenesis in the Fat compared to the Lean line. Therefore the in vivo flux in lipogenesis was measured in both liver and gonadal fat pad (GFP) tissues of males at 5 and 10 weeks of age, using the rate of incorporation of 3H from 3H2O and 14C from acetate and citrate into total lipids. At both ages and in both tissues the Fat line had a higher flux, about 20% increase in the liver and up to three-fold increase (range 1.2- to 3.4-fold) in the GFP. We conclude that direct selection for fatness in mice has resulted in metabolic changes in the rate of de novo fatty acid synthesis, and that the changes are largely detectable before 10 weeks, the age of selection.
在10周龄时经过26代选择后脂肪含量存在显著差异的小鼠品系中，对从头脂肪酸合成途径的通量进行了估计。之前对脂肪生成酶活性的估计表明，与瘦品系相比，肥胖品系的脂肪生成能力有所增加。因此，在5周龄和10周龄雄性小鼠的肝脏和性腺脂肪垫（GFP）组织中，利用3H2O中的3H以及乙酸盐和柠檬酸盐中的14C掺入总脂质的速率，测量了体内脂肪生成的通量。在两个年龄段以及两种组织中，肥胖品系的通量都更高，肝脏中的通量增加约20%，GFP中的通量增加高达三倍（范围为1.2至3.4倍）。我们得出结论，对小鼠肥胖进行直接选择已导致从头脂肪酸合成速率发生代谢变化，并且这些变化在10周龄（选择年龄）之前在很大程度上就可以检测到。
