新型肽序列（“IQ 标签”）与近红外荧光染料具有高亲和力，可用于体内成像的蛋白质和细胞特异性标记。
Novel peptide sequence ("IQ-tag") with high affinity for NIR fluorochromes allows protein and cell specific labeling for in vivo imaging.
机构信息
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America.
出版信息
PLoS One. 2007 Jul 25;2(7):e665. doi: 10.1371/journal.pone.0000665.
BACKGROUND
Probes that allow site-specific protein labeling have become critical tools for visualizing biological processes.
METHODS
Here we used phage display to identify a novel peptide sequence with nanomolar affinity for near infrared (NIR) (benz)indolium fluorochromes. The developed peptide sequence ("IQ-tag") allows detection of NIR dyes in a wide range of assays including ELISA, flow cytometry, high throughput screens, microscopy, and optical in vivo imaging.
SIGNIFICANCE
The described method is expected to have broad utility in numerous applications, namely site-specific protein imaging, target identification, cell tracking, and drug development.
背景
能够实现定点蛋白质标记的探针已成为可视化生物过程的关键工具。
方法
在这里，我们使用噬菌体展示技术来鉴定一种对近红外（NIR）（苯并）吲哚荧光染料具有纳摩尔亲和力的新型肽序列。开发的肽序列（“IQ 标签”）允许在包括 ELISA、流式细胞术、高通量筛选、显微镜和光学体内成像在内的广泛分析中检测 NIR 染料。
意义
所描述的方法预计在许多应用中具有广泛的用途，即定点蛋白质成像、靶标识别、细胞跟踪和药物开发。
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