Zhong F C, Zhen Z J
Department of Forensic Pathology, Sun Yat-sen University of Medical Sciences, Guangzhou, China.
Forensic Sci Int. 1991 Oct;51(2):163-71. doi: 10.1016/0379-0738(91)90182-i.
Localization and estimation of the histamine (HA) content in skin wound edges in 86 Sprague-Dawley rats and three cases of human injuries were carried out by a microfluorimetric method specific for this amine which forms a complex with o-phthalaldehyde (OPT). Distribution and density of the mast cells in the same areas were observed at the same time by toluidine blue stain. In all skin specimens with antemortem wounds, both the epidermis and upper dermis exhibit extracellular yellowish fluorescence of the HA-OPT complex. The fluorescent zone spreads in the wound edges with the lapse of time in vital injuries. The HA content increases gradually up to 30 min and then the yellow histamine fluorescence in areas 0-200 microns from the wound edge decreases. None of these features can be observed in normal skin and postmortem-injured skin. Mast cell degranulation can be demonstrated in all antemortem-injured skin. No statistical relationship exists between the number of mast cells and the HA-OPT fluorescence in either ante- or postmortem-injured groups. This study indicates that skin HA microfluorimetry by the OPT method is of practical value for distinguishing ante- from the postmortem wounds and for timing antemortem wounds.
采用一种针对组胺（HA）的微荧光测定法，该方法利用组胺与邻苯二甲醛（OPT）形成复合物的特性，对86只Sprague-Dawley大鼠的皮肤伤口边缘及3例人类损伤处的HA含量进行了定位和测定。同时，通过甲苯胺蓝染色观察相同区域肥大细胞的分布和密度。在所有生前有伤口的皮肤标本中，表皮和真皮上层均呈现出HA-OPT复合物的细胞外淡黄色荧光。在生前损伤的伤口中，随着时间的推移，荧光区在伤口边缘扩散。HA含量在30分钟内逐渐增加，然后伤口边缘0 - 200微米区域内的黄色组胺荧光减弱。在正常皮肤和死后损伤的皮肤中均未观察到这些特征。在所有生前损伤的皮肤中均可显示肥大细胞脱颗粒现象。在生前或死后损伤组中，肥大细胞数量与HA-OPT荧光之间均无统计学关系。本研究表明，采用OPT法进行皮肤HA微荧光测定对于区分生前伤口和死后伤口以及确定生前伤口的时间具有实际价值。
