Liu M, Wu J, Zhang Z, Li J
Department of Forensic Pathology, Chongqing University of Medical Sciences.
Hua Xi Yi Ke Da Xue Xue Bao. 1995 Dec;26(4):420-3.
Fourty antemortem and 30 postmortem gunshot wound samples of human skin were studied by immunohistochemical method. The 5-HT was seen mainly in wound edge, papillary layer, hypodermis and surrounding tissue of all antemortem gunshot wounds. The 5-HT was also discovered on the postmortem gunshot wounds which occurred within 8 minutes after death. The result showed that immunohistochemical staining of 5-HT be useful for diagnosing the antemortem gunshot wound. It also demonstrated that the mast cells of human do not contain 5-HT. We also studied the rate of mast cell degranulation of gunshot wounds of human skin by toluidine blue staining. The rate of mast cell degranulation of antemortem gunshot wounds increased (50%), and it was higher than that of postmortem gunshot wounds, suggesting that the rising of degranulation rate is a sign of antemortem injury.
采用免疫组织化学方法对40例生前和30例死后人体皮肤枪伤样本进行了研究。5-羟色胺主要见于所有生前枪伤的伤口边缘、乳头层、皮下组织及周围组织。在死后8分钟内发生的死后枪伤上也发现了5-羟色胺。结果表明，5-羟色胺的免疫组织化学染色有助于诊断生前枪伤。还证明人类肥大细胞不含5-羟色胺。我们还通过甲苯胺蓝染色研究了人体皮肤枪伤的肥大细胞脱颗粒率。生前枪伤的肥大细胞脱颗粒率增加（50%），且高于死后枪伤，提示脱颗粒率升高是生前损伤的一个标志。
