de Rijke Eva, Out Pieter, Niessen Wilfried M A, Ariese Freek, Gooijer Cees, Brinkman Udo A Th
Quest International, Department of Analytical Research and Development, Huizerstraatweg 28, 1411 GP Naarden, The Netherlands.
J Chromatogr A. 2006 Apr 21;1112(1-2):31-63. doi: 10.1016/j.chroma.2006.01.019. Epub 2006 Feb 14.
Flavonoids receive considerable attention in the literature, specifically because of their biological and physiological importance. This review focuses on separation and detection methods for flavonoids and their application to plants, food, drinks and biological fluids. The topics that will be discussed are sample treatment, column liquid chromatography (LC), but also methods such as gas chromatography (GC), capillary electrophoresis (CE) and thin-layer chromatography (TLC), various detection methods and structural characterization. Because of the increasing interest in structure elucidation of flavonoids, special attention will be devoted to the use of tandem-mass spectrometric (MS/MS) techniques for the characterization of several important sub-classes, and to the potential of combined diode-array UV (DAD UV), tandem-MS and nuclear magnetic resonance (NMR) detection for unambiguous identification. Emphasis will be on recent developments and trends.
黄酮类化合物在文献中受到了广泛关注，特别是因其生物学和生理学重要性。本综述聚焦于黄酮类化合物的分离与检测方法及其在植物、食品、饮料和生物流体中的应用。将讨论的主题包括样品处理、柱液相色谱（LC），还有气相色谱（GC）、毛细管电泳（CE）和薄层色谱（TLC）等方法、各种检测方法以及结构表征。由于对黄酮类化合物结构解析的兴趣日益增加，将特别关注串联质谱（MS/MS）技术在几个重要亚类表征中的应用，以及二极管阵列紫外（DAD UV）、串联质谱和核磁共振（NMR）联合检测用于明确鉴定的潜力。重点将放在近期的发展和趋势上。
