Georges M, Gunawardana A, Threadgill D W, Lathrop M, Olsaker I, Mishra A, Sargeant L L, Schoeberlein A, Steele M R, Terry C
Genmark Inc., Salt Lake City, Utah 84108.
Genomics. 1991 Sep;11(1):24-32. doi: 10.1016/0888-7543(91)90098-y.
Screening purpose-built libraries with minisatellite probes, we have isolated 36 bovine variable number of tandem repeat markers (VNTRs) characterized by a mean heterozygosity of 59.3 within the American Holstein breed. Matching probabilities and exclusion powers were estimated by Monte-Carlo simulation, showing that the top 5 to 10 markers could be used as a very efficient DNA-based system for individual identification and paternity diagnosis. The isolated VNTR systems should contribute significantly to the establishment of a bovine primary DNA marker map. Linkage analysis, use of somatic cell hybrids, and in situ hybridization demonstrate that these bovine VNTRs are scattered throughout the bovine genome, without evidence for proterminal confinement as in the human, and that at least some of them are organized as clusters. Moreover, Southern blot analysis and in situ hybridization demonstrate conservation of sequence and map location of minisatellites within Bovidae.
通过用小卫星探针筛选专门构建的文库，我们在美国荷斯坦奶牛品种中分离出了36个牛可变串联重复序列标记（VNTRs），其平均杂合度为59.3。通过蒙特卡洛模拟估计了匹配概率和排除能力，结果表明前5至10个标记可作为一种非常有效的基于DNA的个体识别和亲子鉴定系统。分离出的VNTR系统应会对牛初级DNA标记图谱的建立做出重大贡献。连锁分析、体细胞杂种的使用以及原位杂交表明，这些牛VNTRs分散在整个牛基因组中，没有像人类那样存在近端限制的证据，并且其中至少有一些是成簇组织的。此外，Southern印迹分析和原位杂交表明牛科动物中小卫星序列和图谱位置的保守性。
