牛热休克蛋白70基因的染色体定位
Chromosomal localization of HSP70 genes in cattle.
作者信息
Gallagher D S, Grosz M D, Womack J E, Skow L C
机构信息
Department of Veterinary Pathobiology, Texas A&M University, College Station 77843.
出版信息
Mamm Genome. 1993;4(7):388-90. doi: 10.1007/BF00360590.
Five genomic clones representing three HSP70 genes of cattle were biotin labeled and independently hybridized to cattle chromosomes. Fluorescence in situ hybridization localized HSP70-2 to Chromosome (Chr) 23 band 22 (the BoLA region), HSP70-3 to Chr 10 band 34, and HSP70-4 to Chr 3 band 13. Since HSP70-1, a fourth HSP70 gene, is tightly linked with HSP70-2 and the BOLA, HSP70-1 was also localized to Chr 23 band 22. The localization of HSP70-4 is the first assignment of a cattle U6 marker; thus, this entire syntenic group is tentatively placed in cattle Chr 3.
代表牛的三个热休克蛋白70（HSP70）基因的五个基因组克隆用生物素标记，并分别与牛染色体杂交。荧光原位杂交将HSP70-2定位到23号染色体22带（牛主要组织相容性复合体区域），HSP70-3定位到10号染色体34带，HSP70-4定位到3号染色体13带。由于第四个HSP70基因HSP70-1与HSP70-2和牛主要组织相容性复合体紧密连锁，HSP70-1也定位到23号染色体22带。HSP70-4的定位是牛U6标记的首次定位；因此，这个完整的同线群暂时定位在牛3号染色体上。
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