Yamakawa K, Takahashi E, Saito H, Sato T, Oshimura M, Hori T, Nakamura Y
Division of Biochemistry, Cancer Institute, Tokyo, Japan.
Genomics. 1991 Mar;9(3):536-43. doi: 10.1016/0888-7543(91)90421-a.
We have isolated and mapped 75 new DNA markers including 52 restriction fragment length polymorphism (RFLP) markers on human chromosome 3. Clones were mapped by nonisotopic in situ hybridization, in which discrete fluorescent signals can be detected on prometaphase R-banded chromosomes. Thirty-seven markers were mapped to each arm of chromosome 3, and one was localized to the centromere. Five markers defined variable number of tandem repeat (VNTR) loci. Although the 75 clones were scattered throughout the chromosome, they were concentrated in the R-positive bands. This physical map of chromosome 3 will contribute to the characterization of the chromosomal and molecular aberrations involved in renal cell carcinoma, small-cell lung cancer, and other malignancies and in single-gene disorders such as von Hippel-Lindau disease and autosomal dominant retinitis pigmentosa.
我们已经分离并定位了75个新的DNA标记，其中包括位于人类3号染色体上的52个限制性片段长度多态性（RFLP）标记。通过非同位素原位杂交对克隆进行定位，在前中期R带染色体上可检测到离散的荧光信号。37个标记被定位到3号染色体的每条臂上，1个标记定位于着丝粒。5个标记定义了可变串联重复序列（VNTR）位点。尽管这75个克隆分散在整个染色体上，但它们集中在R阳性带中。3号染色体的这一物理图谱将有助于表征与肾细胞癌、小细胞肺癌和其他恶性肿瘤以及诸如冯·希佩尔-林道病和常染色体显性视网膜色素变性等单基因疾病相关的染色体和分子畸变。
