Drinkwater R D, Johnson S E, Barendse W, Harrison B, Womack J E, Hetzel D J
CSIRO, Division of Tropical Animal Production, Gehrmann Laboratories, University of Queensland, Brisbane, Australia.
Genomics. 1994 Jan 1;19(1):149-51. doi: 10.1006/geno.1994.1025.
A minisatellite sequence (RD1613) was isolated from a bovine cosmid genomic library and its chromosomal location determined in cattle and sheep. In cattle, somatic cell hybrid panel analysis assigned RD1613 to the syntenic group U10 with a concordancy of 100%. In situ hybridization placed RD1613 onto bovine chromosome 1 in the region of bands q36-qter. This is the first in situ localization to chromosome 1 in cattle and allows the provisional assignment of syntenic group U10 to this chromosome. It was also found that RD1613 hybridized strongly to sheep genomic DNA. In situ hybridization localized RD1613 to sheep chromosome 1q36-qter, which is consistent with homology between cattle chromosome 1 and sheep chromosome 1q. The RD1613 probe detects a polymorphic single locus marker (designated D1S1) in both cattle and sheep and will be very useful in linkage studies.
从牛黏粒基因组文库中分离出一个小卫星序列（RD1613），并确定了其在牛和绵羊中的染色体定位。在牛中，体细胞杂种板分析将RD1613分配到同线群U10，一致性为100%。原位杂交将RD1613定位到牛1号染色体q36-qter带区。这是首次在牛中定位到1号染色体，并允许将同线群U10临时分配到该染色体。还发现RD1613与绵羊基因组DNA强烈杂交。原位杂交将RD1613定位到绵羊1号染色体1q36-qter，这与牛1号染色体和绵羊1号染色体1q之间的同源性一致。RD1613探针在牛和绵羊中均检测到一个多态性单基因座标记（命名为D1S1），在连锁研究中将非常有用。
