Peng Jinyong, Dong Fuqiu, Qi Yan, Han Xu, Xu Youwei, Xu Lina, Xu Qiwei, Liu Kexin, Zhu Zhenyu
School of Pharmacy, Dalian Medical University, Dalian 116027, People's Republic of China.
Phytochem Anal. 2008 May-Jun;19(3):212-7. doi: 10.1002/pca.1011.
Four triterpene saponins, including astragaloside IV, astragaloside II, astragaloside I and acetylastragaloside I, were successfully isolated and separated by high-speed counter-current chromatography coupled with evaporative light scattering detection from Radix Astragali using stepwise elution with a pair of solvent systems composed of n-hexane:ethyl acetate:ethanol:water in volume ratios of 1:0.6:0.6:1 and 1:1:1:1 (by volume). The isolation produced 26.5 mg astragaloside IV, 28.2 mg astragaloside II, 48.7 mg astragaloside I and 17.6 mg acetylastragaloside I with purities of 97.6, 96.4, 98.8 and 96.8%, respectively, determined by high-performance liquid chromatography from 250 mg crude extract. The chemical structures of the isolated compounds were identified by UV, NMR and MS, and confirmed by authentic standards.
采用高速逆流色谱结合蒸发光散射检测法，以正己烷：乙酸乙酯：乙醇：水体积比为1:0.6:0.6:1和1:1:1:1（体积比）的一对溶剂系统进行梯度洗脱，从黄芪中成功分离出4种三萜皂苷，包括黄芪甲苷IV、黄芪甲苷II、黄芪甲苷I和乙酰黄芪甲苷I。从250 mg粗提物中分离得到26.5 mg黄芪甲苷IV、28.2 mg黄芪甲苷II、48.7 mg黄芪甲苷I和17.6 mg乙酰黄芪甲苷I，通过高效液相色谱法测定其纯度分别为97.6%、96.4%、98.8%和96.8%。通过紫外光谱、核磁共振和质谱对分离得到的化合物的化学结构进行了鉴定，并经标准品确证。
