Xiao Weihua, Han Lujia, Shi Bo
College of Engineering, China Agricultural University, Beijing 100083, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Mar 15;877(8-9):697-702. doi: 10.1016/j.jchromb.2009.01.034. Epub 2009 Jan 31.
High-speed counter-current chromatography methods, combined with resin chromatography were applied to the separation and purification of flavonoid glycosides from the Chinese medicinal herb, Radix Astragali. Five flavonoid glycosides, namely calycosin-7-O-beta-d-glucoside, ononin, (6aR, 11aR)-9,10-dimethoxypterocarpan-3-O-beta-d-glucoside, (3R)-2'-hydroxy-3',4'-dimethoxyisoflavan-7-O-beta-d-glucoside and calycosin-7-O-beta-d-glucoside-6''-O-acetate, were obtained. Among them, calycosin-7-O-beta-d-glucoside-6''-O-acetate was preparatively separated from Radix Astragali for the first time. Their structures were identified by ESI-MS, (1)H NMR, (13)C NMR, and 2D NMR.
采用高速逆流色谱法结合树脂色谱法，从中药黄芪中分离纯化黄酮苷。得到了5种黄酮苷，分别为毛蕊异黄酮-7-O-β-D-葡萄糖苷、芒柄花苷、(6aR,11aR)-9,10-二甲氧基紫檀烷-3-O-β-D-葡萄糖苷、(3R)-2'-羟基-3',4'-二甲氧基异黄酮-7-O-β-D-葡萄糖苷和毛蕊异黄酮-7-O-β-D-葡萄糖苷-6''-O-乙酸酯。其中，毛蕊异黄酮-7-O-β-D-葡萄糖苷-6''-O-乙酸酯首次从黄芪中制备分离得到。通过电喷雾电离质谱（ESI-MS）、氢核磁共振（¹H NMR）、碳核磁共振（¹³C NMR）和二维核磁共振（2D NMR）对其结构进行了鉴定。
