Kostanyan Artak A, Voshkin Andrey A, Belova Vera V
Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, 31 Leninskii pr., 119991 Moscow, Russia.
Molecules. 2020 Dec 18;25(24):6020. doi: 10.3390/molecules25246020.
Countercurrent liquid-liquid chromatographic techniques (CCC), similar to solvent extraction, are based on the different distribution of compounds between two immiscible liquids and have been most widely used in natural product separations. Due to its high load capacity, low solvent consumption, the diversity of separation methods, and easy scale-up, CCC provides an attractive tool to obtain pure compounds in the analytical, preparative, and industrial-scale separations. This review focuses on the steady-state and non-steady-state CCC separations ranging from conventional CCC to more novel methods such as different modifications of dual mode, closed-loop recycling, and closed-loop recycling dual modes. The design and modeling of various embodiments of CCC separation processes have been described.
逆流液-液色谱技术（CCC）与溶剂萃取类似，基于化合物在两种不互溶液体之间的不同分配，已在天然产物分离中得到最广泛的应用。由于其高负载能力、低溶剂消耗、分离方法的多样性以及易于放大，CCC为在分析、制备和工业规模分离中获得纯化合物提供了一个有吸引力的工具。本综述重点关注从传统CCC到更新颖方法（如双模的不同改进、闭环循环和闭环循环双模）的稳态和非稳态CCC分离。已描述了CCC分离过程各种实施方案的设计和建模。
