de la Fuente José, Ruiz-Fons Francisco, Naranjo Victoria, Torina Alessandra, Rodríguez Oscar, Gortázar Christian
Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13071 Ciudad Real, Spain.
Res Vet Sci. 2008 Jun;84(3):382-6. doi: 10.1016/j.rvsc.2007.05.018. Epub 2007 Jul 25.
Anaplasma spp. (Rickettsiales: Anaplasmataceae) are tick-borne pathogens of veterinary and human importance. The wildlife hosts for these pathogens are not well characterized and may play an important role in the epidemiology of the disease. The objective of this research was to study the infection with A. marginale, A. ovis and A. phagocytophilum in free-ranging European roe deer (Capreolus capreolus) from Cádiz, Andalucía, Spain. Of 17 roe deer tested, 14 (82%) and 5 (29%) had antibodies reactive to Anaplasma spp. and A. phagocytophilum by competitive ELISA and indirect immunofluorescent antibody testing, respectively. Polymerase chain reaction and sequence analysis of Anaplasma major surface protein 4 (msp4) gene was conducted on blood samples from all roe deer examined. Nine (53%) animals had evidence of infection with A. ovis and 3 (18%) were positive for A. phagocytophilum. Concurrent infections were not detected. Despite the presence of A. marginale infections in cattle in the study site (36% msp4 PCR-positive animals), none of the msp4 amplicons from roe deer corresponded to A. marginale sequences. A. ovis msp4 sequences were identical to a genotype previously identified in sheep in Sicily, Italy. Two different A. phagocytophilum genotypes were identified in infected roe deer. This is the first report of roe deer naturally infected with A. ovis. These results demonstrate that roe deer are infected with A. ovis and A. phagocytophilum in Spain and suggest that this species may be involved in the natural cycle of these pathogens in this region, thus acting as potential reservoir for transmission to domestic and wild animals.
无形体属（立克次氏体目：无形体科）是对兽医和人类具有重要意义的蜱传病原体。这些病原体的野生动物宿主尚未得到充分描述，可能在该疾病的流行病学中发挥重要作用。本研究的目的是研究西班牙安达卢西亚加的斯自由放养的欧洲狍（Capreolus capreolus）中边缘无形体、绵羊无形体和嗜吞噬细胞无形体的感染情况。在检测的17只狍中，分别有14只（82%）和5只（29%）通过竞争ELISA和间接免疫荧光抗体检测，其抗体对无形体属和嗜吞噬细胞无形体呈反应性。对所有检测狍的血样进行了无形体主要表面蛋白4（msp4）基因的聚合酶链反应和序列分析。9只（53%）动物有绵羊无形体感染的证据，3只（18%）嗜吞噬细胞无形体呈阳性。未检测到混合感染。尽管研究地点的牛群中存在边缘无形体感染（36%的动物msp4 PCR检测呈阳性），但狍的msp4扩增子均与边缘无形体序列不符。绵羊无形体msp4序列与先前在意大利西西里岛绵羊中鉴定的一个基因型相同。在感染的狍中鉴定出两种不同的嗜吞噬细胞无形体基因型。这是关于狍自然感染绵羊无形体的首次报告。这些结果表明，西班牙的狍感染了绵羊无形体和嗜吞噬细胞无形体，提示该物种可能参与了这些病原体在该地区的自然循环，从而成为向家畜和野生动物传播的潜在宿主。
