Kadyrova Madina, Ostrovskii Alexandr, Mukanov Kassym, Kassen Amirkhan, Shevtsova Elena, Berdikulov Maxat, Vergnaud Gilles, Shevtsov Alexandr
Laboratory of Applied Genetics, National Center for Biotechnology, Astana 010000, Kazakhstan.
National Veterinary Reference Center, 150 let Abaya Street 22/3, Astana 010000, Kazakhstan.
Pathogens. 2024 Oct 12;13(10):894. doi: 10.3390/pathogens13100894.
Bovine anaplasmosis is an infectious vector-borne disease caused by bacteria of the genus , which have a wide global distribution and represent a high economic burden for agriculture. The use of molecular genetic techniques has increased our knowledge of the species diversity of spp. and naturally susceptible animals. Monitoring studies allow us to assess the level of infection in herds, as well as the involvement of natural vectors in the processes of maintaining and spreading infection. Despite the high prevalence of and in cattle in Kazakhstan, there is no information on the distribution and species diversity of spp in this country. As part of this work, 7027 DNA samples isolated from the whole blood of cattle from 175 settlements in all 17 Kazakhstan regions were PCR-tested for the presence of spp. carriers were found in 1.3% (90 out of 7027) of the tested animals in 9 of the 17 regions of Kazakhstan. The highest percentage of infected animals was recorded in Turkistan (South Kazakhstan) and North Kazakhstan with 4.46% and 2.48% positive samples, respectively. The partial sequencing of and the gene allowed us to identify five species of : , , Mongolica, , and Unknown with infection rates of 0.63%, 0.44%, 0.13%, 0.01%, and 0.01%, respectively.
牛无形体病是由无形体属细菌引起的一种传染性媒介传播疾病，该属细菌在全球分布广泛，给农业带来了巨大的经济负担。分子遗传技术的应用增加了我们对无形体物种多样性以及自然易感动物的了解。监测研究使我们能够评估畜群中的感染水平，以及自然媒介在感染维持和传播过程中的作用。尽管哈萨克斯坦牛群中无形体和嗜吞噬细胞无形体的感染率很高，但该国关于无形体物种分布和多样性的信息却很缺乏。作为这项工作的一部分，对从哈萨克斯坦所有17个地区175个定居点的牛全血中分离出的7027份DNA样本进行了PCR检测，以确定无形体属细菌的存在情况。在哈萨克斯坦17个地区中的9个地区，1.3%（7027份样本中有90份）的检测动物被发现为无形体属细菌携带者。感染动物比例最高的是突厥斯坦（南哈萨克斯坦）和北哈萨克斯坦，阳性样本率分别为4.46%和2.48%。对16S rRNA和groEL基因进行部分测序使我们能够鉴定出无形体属的五个物种：嗜吞噬细胞无形体、牛无形体、蒙古无形体、边缘无形体和未知无形体，感染率分别为0.63%、0.44%、0.13%、0.01%和0.01%。
