Li Deng-Feng, Zhang Ming-Chang, Yang Hai-Jie, Zhu Yan-Bing, Xu Xun
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, P.R. China.
Virology. 2007 Nov 10;368(1):122-32. doi: 10.1016/j.virol.2007.06.027. Epub 2007 Jul 25.
White Spot Syndrome Virus (WSSV) is a virulent and widespread dsDNA virus with a wide range of hosts. Although remarkable progress has been made on virus characterization, however, its mechanism of infection is poorly understood. In this study, by analyzing the phage display library of the WSSV genome, a WSSV envelope protein VP187 (wsv209) was found to interact with shrimp integrin. VP187 possesses the RGD motif. The interaction between integrin and VP187 was confirmed with coimmunoprecipitation. These results demonstrate for the first time an interaction between the WSSV envelope protein and a cell surface molecule. Soluble integrin, integrin-specific antibody and an RGD-containing peptide were found to block the WSSV infection in vivo and in vitro. Gene silencing using a sequence-specific dsRNA targeting beta-integrin effectively inhibited the virus infection. These findings suggest that beta-integrin may function as a cellular receptor for WSSV infection.
白斑综合征病毒（WSSV）是一种毒性强且广泛传播的双链DNA病毒，宿主范围广泛。尽管在病毒特性研究方面取得了显著进展，但其感染机制仍知之甚少。在本研究中，通过分析WSSV基因组的噬菌体展示文库，发现一种WSSV包膜蛋白VP187（wsv209）与虾整合素相互作用。VP187具有RGD基序。通过共免疫沉淀证实了整合素与VP187之间的相互作用。这些结果首次证明了WSSV包膜蛋白与细胞表面分子之间的相互作用。发现可溶性整合素、整合素特异性抗体和含RGD的肽在体内和体外均可阻断WSSV感染。使用靶向β-整合素的序列特异性双链RNA进行基因沉默可有效抑制病毒感染。这些发现表明β-整合素可能作为WSSV感染的细胞受体发挥作用。
