Papoian R, Duffy F, Sanner M, Wilt E
Department of Biopharmaceutics, Sandoz Pharma, Ltd., Basel, Switzerland.
J Immunol Methods. 1991 Dec 15;145(1-2):161-5. doi: 10.1016/0022-1759(91)90322-7.
We describe a sensitive ELISA for the measurement, of the recombinant human cytokine, interleukin-3 (IL-3), in human plasma or serum samples. The assay design uses two different anti-IL-3 monoclonal antibodies, giving a two-site assay configuration. The assay incorporates the use of alkaline phosphatase conjugated to streptavidin and a biotinylated anti-IL-3 monoclonal antibody to amplify the resultant signal. This ELISA can measure both glycosylated and non-glycosylated IL-3. The limits of quantification, as determined by precision profiles and quality control samples prepared in 100% human plasma, are 20 pg/ml and 30 pg/ml for non-glycosylated and glycosylated IL-3, respectively.
我们描述了一种灵敏的酶联免疫吸附测定法（ELISA），用于测量人血浆或血清样本中重组人细胞因子白细胞介素-3（IL-3）。该检测方法设计使用两种不同的抗IL-3单克隆抗体，形成双位点检测结构。该检测采用与链霉亲和素偶联的碱性磷酸酶和生物素化抗IL-3单克隆抗体来放大所得信号。这种ELISA可以检测糖基化和非糖基化的IL-3。通过精密度曲线和在100%人血浆中制备的质量控制样品确定，非糖基化和糖基化IL-3的定量限分别为20 pg/ml和30 pg/ml。
