骨的电子显微镜检查

Electron microscopy of bone.

作者信息

Everts V, Hoeben K A

机构信息

Department of Oral Cell Biology, Academic Centre of Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, The Netherlands.

出版信息

Methods Cell Biol. 2013;113:59-79. doi: 10.1016/B978-0-12-407239-8.00004-5.

DOI:10.1016/B978-0-12-407239-8.00004-5

PMID:23317898

Abstract

Mineralized tissues like bone, dentin and mineralized cartilage are difficult to prepare for ultrastructural analysis. In general, the higher the level of mineralization is, the more difficult it is to obtain ultrathin sections of these tissue. Tissues with a low level of mineral, e.g. from young animals, are rather simple to prepare and sectioning is not that much of a problem. In the present chapter we describe step-by-step how to prepare mineralized tissues for ultrastructural examination.

摘要

像骨骼、牙本质和矿化软骨这样的矿化组织很难制备用于超微结构分析。一般来说，矿化程度越高，获取这些组织的超薄切片就越困难。矿化程度低的组织，例如来自幼龄动物的组织，制备起来相当简单，切片也不是什么大问题。在本章中，我们将逐步描述如何制备矿化组织用于超微结构检查。