骨的电子显微镜检查

Electron microscopy of bone.

作者信息

Everts V, Hoeben K A

机构信息

Department of Oral Cell Biology, Academic Centre of Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, The Netherlands.

出版信息

Methods Cell Biol. 2013;113:59-79. doi: 10.1016/B978-0-12-407239-8.00004-5.

DOI:10.1016/B978-0-12-407239-8.00004-5

Abstract

Mineralized tissues like bone, dentin and mineralized cartilage are difficult to prepare for ultrastructural analysis. In general, the higher the level of mineralization is, the more difficult it is to obtain ultrathin sections of these tissue. Tissues with a low level of mineral, e.g. from young animals, are rather simple to prepare and sectioning is not that much of a problem. In the present chapter we describe step-by-step how to prepare mineralized tissues for ultrastructural examination.

摘要

像骨骼、牙本质和矿化软骨这样的矿化组织很难制备用于超微结构分析。一般来说，矿化程度越高，获取这些组织的超薄切片就越困难。矿化程度低的组织，例如来自幼龄动物的组织，制备起来相当简单，切片也不是什么大问题。在本章中，我们将逐步描述如何制备矿化组织用于超微结构检查。

相似文献

1

Electron microscopy of bone.骨的电子显微镜检查

Methods Cell Biol. 2013;113:59-79. doi: 10.1016/B978-0-12-407239-8.00004-5.

2

Transmission electron microscopy of bone.骨的透射电子显微镜检查

Methods Mol Biol. 2012;816:351-63. doi: 10.1007/978-1-61779-415-5_23.

3

Demineralized Murine Skeletal Histology.脱钙鼠骨骼组织学。

Methods Mol Biol. 2021;2230:283-302. doi: 10.1007/978-1-0716-1028-2_16.

4

Microwave-assisted processing and embedding for transmission electron microscopy.用于透射电子显微镜的微波辅助处理与包埋

Methods Mol Biol. 2007;369:47-65. doi: 10.1007/978-1-59745-294-6_4.

5

Transmission electron microscopy of cartilage and bone.软骨和骨的透射电子显微镜检查

Methods Cell Biol. 2010;96:443-73. doi: 10.1016/S0091-679X(10)96019-6.

6

Conventional specimen preparation techniques for transmission electron microscopy of cultured cells.用于培养细胞透射电子显微镜检查的传统标本制备技术。

Methods Mol Biol. 2007;369:1-18. doi: 10.1007/978-1-59745-294-6_1.

7

Demineralized murine skeletal histology.脱矿质小鼠骨骼组织学。

Methods Mol Biol. 2014;1130:165-183. doi: 10.1007/978-1-62703-989-5_12.

8

Pre- and Post-embedding Immunogold Labeling of Tissue Sections.组织切片的包埋前和包埋后免疫金标记

Methods Mol Biol. 2016;1474:291-307. doi: 10.1007/978-1-4939-6352-2_19.

9

Pre-embedding Nanogold Silver and Gold Intensification.包埋前纳米金银及金增强法。

Methods Mol Biol. 2016;1474:269-78. doi: 10.1007/978-1-4939-6352-2_17.

10

Preparation of mineralized tissue for light microscopy.用于光学显微镜检查的矿化组织制备

Methods Mol Biol. 2012;915:37-50. doi: 10.1007/978-1-61779-977-8_3.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。