Bharathi M J, Ramakrishnan R, Vasu S, Palaniappan R
Department of Microbiology, Aravind Eye Hospital and Postgraduate Institute of Ophthalmology, Tirunelveli - 627 001, India.
Indian J Med Microbiol. 2002 Jan-Mar;20(1):19-24.
To identify the specific microbial pathogens responsible for corneal ulceration in South India and compare these profiles with other series.
All patients with infectious keratitis who presented between 20th September 1999 and 31st March 2001 were evaluated. They were examined by slit-lamp biomicroscopy and corneal scrapings were performed for cultures and smears by using standard protocols.
In the 18 months period, 1618 patients with corneal ulcerations were evaluated. Corneal cultures were found to be positive in 1126 (69.59%) patients. Of the 1618 patients, 566 (34.98%) had bacterial growth, 522 (32.26%) had fungal growth, 30 (1.85%) had mixed bacterial and fungal growth, 8 (0.49%) had Acanthamoeba species growth and the remaining 492 (30.41%) were found to be culture negative. The predominant bacterial pathogen isolated was Streptococcus pneumoniae representing 41.85%, followed by Pseudomonas aeruginosa 21.25%. The predominant fungal pathogens isolated were Fusarium species (45.85%) followed by Aspergillus species (24.37%).
Bacterial and fungal infections occurred almost with equal frequency, the predominant bacterial and fungal species isolated being Streptococcus pneumoniae and Fusarium species respectively. The findings of our study show that there is a region wise variation in the predominance of corneal pathogens. This has an important public health implication for the initiation of therapy.
确定印度南部导致角膜溃疡的特定微生物病原体，并将这些情况与其他系列研究进行比较。
对1999年9月20日至2001年3月31日期间所有出现感染性角膜炎的患者进行评估。通过裂隙灯生物显微镜检查对他们进行检查，并按照标准方案对角膜刮片进行培养和涂片。
在这18个月期间，对1618例角膜溃疡患者进行了评估。发现1126例（69.59%）患者的角膜培养呈阳性。在这1618例患者中，566例（34.98%）有细菌生长，522例（32.26%）有真菌生长，30例（1.85%）有细菌和真菌混合生长，8例（0.49%）有棘阿米巴属生长，其余492例（30.41%）培养为阴性。分离出的主要细菌病原体是肺炎链球菌，占41.85%，其次是铜绿假单胞菌，占21.25%。分离出的主要真菌病原体是镰刀菌属（45.85%），其次是曲霉菌属（24.37%）。
细菌和真菌感染的发生频率几乎相等，分离出的主要细菌和真菌种类分别是肺炎链球菌和镰刀菌属。我们的研究结果表明，角膜病原体的优势存在区域差异。这对治疗的启动具有重要的公共卫生意义。
