Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection.

作者信息

Ferrari B C, Power M L, Bergquist P L

机构信息

Department of Chemistry and Biomolecular Sciences, Environmental Biotechnology CRC and Biotechnology Research Institute, Macquarie University Sydney, Sydney, NSW, 2109, Australia.

出版信息

Biotechnol Lett. 2007 Dec;29(12):1831-7. doi: 10.1007/s10529-007-9487-2. Epub 2007 Jul 27.

Current DNA extraction methods for parasites are labour-intensive and usually involve several steps, increasing the potential for cross-contamination. We describe here a closed-tube DNA extraction procedure based upon the use of a thermostable proteinase that enabled sensitive amplification of target loci from parasites from diverse lineages including Apicomplexa, Sarcomastgophora and Nematoda. Moreover, this procedure is not subject to cross-contamination and is readily adaptable to automation.