苹果蠹蛾流行期间分离的苏云金芽孢杆菌菌株中cry基因谱分析
Analysis of cry gene profiles in Bacillus thuringiensis strains isolated during epizootics in Cydia pomonella L.
作者信息
Konecka Edyta, Kaznowski Adam, Ziemnicka Jadwiga, Ziemnicki Kazimierz, Paetz Halina
机构信息
Department of Microbiology, Institute of Experimental Biology, Adam Mickiewicz University, Fredry 10, 61-701, Poznań, Poland.
出版信息
Curr Microbiol. 2007 Sep;55(3):217-22. doi: 10.1007/s00284-007-0085-2. Epub 2007 Jul 25.
The crystal morphology and the profiles of genes encoding protein toxins (Cry and Cyt) were analyzed in 12 Bacillus thuringiensis strains isolated during epizootics in laboratory culture lines of Cydia pomonella, 2 isolates cultured from Leucoma salicis larvae, and 9 reference strains. Epizootic isolates produced crystals of the same bipyramidal shape; however, they revealed a variety of number and type of cry genes. Genes cry1I, cry2Ab, and cry9B were the most frequently observed in epizootic strains. Gene cry1I was noted in of 50% epizootic isolates. Eighty-three percent of them harbored gene cry2Ab. Gene cry9B was found for 42% of strains isolated during epizootics. Three isolates showed the largest number of cry genes and their variety; hence, they were chosen for the toxicity assay of their crystals and spores on C. pomonella larvae. One of them had approximately sixfold higher insecticidal activity than the reference strain B. thuringiensis subsp. kurstaki BTK STANDARD.
对从苹果蠹蛾实验室培养品系的流行病中分离出的12株苏云金芽孢杆菌菌株、从柳毒蛾幼虫中培养出的2株分离株以及9株参考菌株，分析了其晶体形态和编码蛋白质毒素（Cry和Cyt）的基因图谱。流行病分离株产生相同双金字塔形状的晶体；然而，它们显示出cry基因数量和类型的多样性。cry1I、cry2Ab和cry9B基因在流行病菌株中最常被观察到。50%的流行病分离株中发现了cry1I基因。其中83%含有cry2Ab基因。在流行病期间分离的菌株中，42%发现了cry9B基因。三个分离株显示出cry基因数量最多且种类多样；因此，选择它们对苹果蠹蛾幼虫进行晶体和孢子的毒性测定。其中一个分离株的杀虫活性比参考菌株苏云金芽孢杆菌库尔斯塔克亚种BTK STANDARD高约六倍。
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